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The hit-and-return system enables efficient time-resolved serial synchrotron crystallography

Standard

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography. / Schulz, Eike C; Mehrabi, Pedram; Müller-Werkmeister, Henrike M; Tellkamp, Friedjof; Jha, Ajay; Stuart, William; Persch, Elke; De Gasparo, Raoul; Diederich, François; Pai, Emil F; Miller, R J Dwayne.

in: NAT METHODS, Jahrgang 15, Nr. 11, 11.2018, S. 901-904.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Schulz, EC, Mehrabi, P, Müller-Werkmeister, HM, Tellkamp, F, Jha, A, Stuart, W, Persch, E, De Gasparo, R, Diederich, F, Pai, EF & Miller, RJD 2018, 'The hit-and-return system enables efficient time-resolved serial synchrotron crystallography', NAT METHODS, Jg. 15, Nr. 11, S. 901-904. https://doi.org/10.1038/s41592-018-0180-2

APA

Schulz, E. C., Mehrabi, P., Müller-Werkmeister, H. M., Tellkamp, F., Jha, A., Stuart, W., Persch, E., De Gasparo, R., Diederich, F., Pai, E. F., & Miller, R. J. D. (2018). The hit-and-return system enables efficient time-resolved serial synchrotron crystallography. NAT METHODS, 15(11), 901-904. https://doi.org/10.1038/s41592-018-0180-2

Vancouver

Schulz EC, Mehrabi P, Müller-Werkmeister HM, Tellkamp F, Jha A, Stuart W et al. The hit-and-return system enables efficient time-resolved serial synchrotron crystallography. NAT METHODS. 2018 Nov;15(11):901-904. https://doi.org/10.1038/s41592-018-0180-2

Bibtex

@article{52a7a72a428f482398114f3f750d7087,
title = "The hit-and-return system enables efficient time-resolved serial synchrotron crystallography",
abstract = "We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.",
keywords = "Crystallography, X-Ray, Equipment Design, Hydrolases/chemistry, Models, Molecular, Protein Conformation, Rhodopseudomonas/enzymology, Synchrotrons/instrumentation, Time Factors",
author = "Schulz, {Eike C} and Pedram Mehrabi and M{\"u}ller-Werkmeister, {Henrike M} and Friedjof Tellkamp and Ajay Jha and William Stuart and Elke Persch and {De Gasparo}, Raoul and Fran{\c c}ois Diederich and Pai, {Emil F} and Miller, {R J Dwayne}",
year = "2018",
month = nov,
doi = "10.1038/s41592-018-0180-2",
language = "English",
volume = "15",
pages = "901--904",
journal = "NAT METHODS",
issn = "1548-7091",
publisher = "NATURE PUBLISHING GROUP",
number = "11",

}

RIS

TY - JOUR

T1 - The hit-and-return system enables efficient time-resolved serial synchrotron crystallography

AU - Schulz, Eike C

AU - Mehrabi, Pedram

AU - Müller-Werkmeister, Henrike M

AU - Tellkamp, Friedjof

AU - Jha, Ajay

AU - Stuart, William

AU - Persch, Elke

AU - De Gasparo, Raoul

AU - Diederich, François

AU - Pai, Emil F

AU - Miller, R J Dwayne

PY - 2018/11

Y1 - 2018/11

N2 - We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

AB - We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

KW - Crystallography, X-Ray

KW - Equipment Design

KW - Hydrolases/chemistry

KW - Models, Molecular

KW - Protein Conformation

KW - Rhodopseudomonas/enzymology

KW - Synchrotrons/instrumentation

KW - Time Factors

U2 - 10.1038/s41592-018-0180-2

DO - 10.1038/s41592-018-0180-2

M3 - SCORING: Journal article

C2 - 30377366

VL - 15

SP - 901

EP - 904

JO - NAT METHODS

JF - NAT METHODS

SN - 1548-7091

IS - 11

ER -