The early expression of VAChT and VIP in mouse sympathetic ganglia is not induced by cytokines acting through LIFRbeta or CNTFRalpha
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The early expression of VAChT and VIP in mouse sympathetic ganglia is not induced by cytokines acting through LIFRbeta or CNTFRalpha. / Stanke, M; Geissen, M; Götz, R; Ernsberger, U; Rohrer, H.
In: MECH DEVELOP, Vol. 91, No. 1-2, 01.03.2000, p. 91-96.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - The early expression of VAChT and VIP in mouse sympathetic ganglia is not induced by cytokines acting through LIFRbeta or CNTFRalpha
AU - Stanke, M
AU - Geissen, M
AU - Götz, R
AU - Ernsberger, U
AU - Rohrer, H
PY - 2000/3/1
Y1 - 2000/3/1
N2 - Sympathetic ganglia consist of noradrenergic and cholinergic neurons. The cholinergic marker protein vesicular acetylcholine transporter (VAChT) and the neuropeptide vasoactive intestinal peptide (VIP), co-expressed in mature cholinergic sympathetic neurons, are first detectable during embryonic development of rat sympathetic ganglia. However, the subpopulation of cholinergic sympathetic neurons which innervates sweat glands in mammalian footpads starts to express VAChT and VIP during the first postnatal weeks, under the influence of sweat gland-derived signals. In vitro evidence suggests that the sweat gland-derived cholinergic differentiation factor belongs to a group of neuropoietic cytokines, including LIF, CNTF and CT-1, that act through a LIFRbeta-containing cytokine receptor. To investigate whether the embryonic expression of cholinergic properties is elicited by a related cytokine, the expression of VAChT and VIP was analyzed in stellate ganglia of mice deficient for the cytokine receptor subunits LIFRbeta or CNTFRalpha. The density of VAChT- and VIP-immunoreactive cells in stellate ganglia of new-born animals was not different in LIFRbeta(-/-) and CNTFRalpha(-/-) ganglia as compared to ganglia from wild-type mice. These results demonstrate that the early, embryonic expression of VAChT and VIP is not induced by cytokines acting through LIFRbeta- or CNTFRalpha-containing receptors.
AB - Sympathetic ganglia consist of noradrenergic and cholinergic neurons. The cholinergic marker protein vesicular acetylcholine transporter (VAChT) and the neuropeptide vasoactive intestinal peptide (VIP), co-expressed in mature cholinergic sympathetic neurons, are first detectable during embryonic development of rat sympathetic ganglia. However, the subpopulation of cholinergic sympathetic neurons which innervates sweat glands in mammalian footpads starts to express VAChT and VIP during the first postnatal weeks, under the influence of sweat gland-derived signals. In vitro evidence suggests that the sweat gland-derived cholinergic differentiation factor belongs to a group of neuropoietic cytokines, including LIF, CNTF and CT-1, that act through a LIFRbeta-containing cytokine receptor. To investigate whether the embryonic expression of cholinergic properties is elicited by a related cytokine, the expression of VAChT and VIP was analyzed in stellate ganglia of mice deficient for the cytokine receptor subunits LIFRbeta or CNTFRalpha. The density of VAChT- and VIP-immunoreactive cells in stellate ganglia of new-born animals was not different in LIFRbeta(-/-) and CNTFRalpha(-/-) ganglia as compared to ganglia from wild-type mice. These results demonstrate that the early, embryonic expression of VAChT and VIP is not induced by cytokines acting through LIFRbeta- or CNTFRalpha-containing receptors.
KW - Animals
KW - Carrier Proteins/metabolism
KW - Ciliary Neurotrophic Factor/metabolism
KW - Growth Inhibitors/metabolism
KW - Interleukin-6
KW - Leukemia Inhibitory Factor
KW - Leukemia Inhibitory Factor Receptor alpha Subunit
KW - Lymphokines/metabolism
KW - Membrane Transport Proteins
KW - Mice
KW - Rabbits
KW - Rats
KW - Receptor, Ciliary Neurotrophic Factor/metabolism
KW - Receptors, Cytokine/metabolism
KW - Receptors, OSM-LIF
KW - Stellate Ganglion/metabolism
KW - Vasoactive Intestinal Peptide/metabolism
KW - Vesicular Acetylcholine Transport Proteins
KW - Vesicular Transport Proteins
U2 - 10.1016/s0925-4773(99)00275-0
DO - 10.1016/s0925-4773(99)00275-0
M3 - SCORING: Journal article
C2 - 10704834
VL - 91
SP - 91
EP - 96
JO - MECH DEVELOP
JF - MECH DEVELOP
SN - 0925-4773
IS - 1-2
ER -