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The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells

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The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells. / Açil, Y; Möller, B; Niehoff, P; Rachko, K; Gassling, V; Wiltfang, J; Simon, M J K.

In: J CRANIO MAXILL SURG, Vol. 40, No. 8, 12.2012, p. e229-35.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Açil, Y, Möller, B, Niehoff, P, Rachko, K, Gassling, V, Wiltfang, J & Simon, MJK 2012, 'The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells', J CRANIO MAXILL SURG, vol. 40, no. 8, pp. e229-35. https://doi.org/10.1016/j.jcms.2011.10.024

APA

Açil, Y., Möller, B., Niehoff, P., Rachko, K., Gassling, V., Wiltfang, J., & Simon, M. J. K. (2012). The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells. J CRANIO MAXILL SURG, 40(8), e229-35. https://doi.org/10.1016/j.jcms.2011.10.024

Vancouver

Açil Y, Möller B, Niehoff P, Rachko K, Gassling V, Wiltfang J et al. The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells. J CRANIO MAXILL SURG. 2012 Dec;40(8):e229-35. https://doi.org/10.1016/j.jcms.2011.10.024

Bibtex

@article{810f2ac4c26a4037ad4a4053634c16a3,
title = "The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells",
abstract = "INTRODUCTION: Osteonecrosis of the jaw (ONJ) is an emerging condition in patients undergoing long-term administration of bisphosphonates (BP) for the treatment of osteoporosis and hypercalcaemia associated with malignancy, multiple myeloma, and metastatic breast and prostate cancers. This is a follow-up study, its purpose was to examine the effects in-vitro of intravenous zoledronic acid (ZOL) and pamidronate (PAM) and oral alendronate (FOS) on the human oral cavity using gingival fibroblasts and osteoblasts cells and, in addition, osteogenic sarcoma cells (SaOS-2-cells).MATERIALS AND METHODS: Human gingival fibroblasts, osteoblasts and SaOS-2-cells were seeded on multiple 6-well plates at a density of 5 × 10(5)cells in a 4-week cell culture. Four different concentrations (1, 5, 10, 20 μM) of each BP (ZOL, PAM, FOS) and pyrophosphate were used in this study.RESULTS: All BP decreased collagen production and lowered cell proliferation in-vitro. ZOL was the component with most inhibitory effect.CONCLUSION: The findings in this study suggest that ZOL, PAM and FOS generally diminish cell proliferation and collagen production of human gingival fibroblasts, osteoblasts and SaOS-2-cells. The present follow-up study shows that not only ZOL and PAM but also FOS have a strong inhibitory effect on collagen production and cell survival in-vitro.",
keywords = "Alendronate, Alkaline Phosphatase, Bone Density Conservation Agents, Cell Line, Tumor, Cell Proliferation, Cell Survival, Cells, Cultured, Chromatography, High Pressure Liquid, Collagen Type I, Coloring Agents, Diphosphates, Diphosphonates, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Fibroblasts, Follow-Up Studies, Gingiva, Humans, Imidazoles, Osteoblasts, Osteocalcin, Osteosarcoma, Tetrazolium Salts, Thiazoles, Journal Article, Research Support, Non-U.S. Gov't",
author = "Y A{\c c}il and B M{\"o}ller and P Niehoff and K Rachko and V Gassling and J Wiltfang and Simon, {M J K}",
note = "Copyright {\textcopyright} 2011 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.",
year = "2012",
month = dec,
doi = "10.1016/j.jcms.2011.10.024",
language = "English",
volume = "40",
pages = "e229--35",
journal = "J CRANIO MAXILL SURG",
issn = "1010-5182",
publisher = "Elsevier",
number = "8",

}

RIS

TY - JOUR

T1 - The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells

AU - Açil, Y

AU - Möller, B

AU - Niehoff, P

AU - Rachko, K

AU - Gassling, V

AU - Wiltfang, J

AU - Simon, M J K

N1 - Copyright © 2011 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

PY - 2012/12

Y1 - 2012/12

N2 - INTRODUCTION: Osteonecrosis of the jaw (ONJ) is an emerging condition in patients undergoing long-term administration of bisphosphonates (BP) for the treatment of osteoporosis and hypercalcaemia associated with malignancy, multiple myeloma, and metastatic breast and prostate cancers. This is a follow-up study, its purpose was to examine the effects in-vitro of intravenous zoledronic acid (ZOL) and pamidronate (PAM) and oral alendronate (FOS) on the human oral cavity using gingival fibroblasts and osteoblasts cells and, in addition, osteogenic sarcoma cells (SaOS-2-cells).MATERIALS AND METHODS: Human gingival fibroblasts, osteoblasts and SaOS-2-cells were seeded on multiple 6-well plates at a density of 5 × 10(5)cells in a 4-week cell culture. Four different concentrations (1, 5, 10, 20 μM) of each BP (ZOL, PAM, FOS) and pyrophosphate were used in this study.RESULTS: All BP decreased collagen production and lowered cell proliferation in-vitro. ZOL was the component with most inhibitory effect.CONCLUSION: The findings in this study suggest that ZOL, PAM and FOS generally diminish cell proliferation and collagen production of human gingival fibroblasts, osteoblasts and SaOS-2-cells. The present follow-up study shows that not only ZOL and PAM but also FOS have a strong inhibitory effect on collagen production and cell survival in-vitro.

AB - INTRODUCTION: Osteonecrosis of the jaw (ONJ) is an emerging condition in patients undergoing long-term administration of bisphosphonates (BP) for the treatment of osteoporosis and hypercalcaemia associated with malignancy, multiple myeloma, and metastatic breast and prostate cancers. This is a follow-up study, its purpose was to examine the effects in-vitro of intravenous zoledronic acid (ZOL) and pamidronate (PAM) and oral alendronate (FOS) on the human oral cavity using gingival fibroblasts and osteoblasts cells and, in addition, osteogenic sarcoma cells (SaOS-2-cells).MATERIALS AND METHODS: Human gingival fibroblasts, osteoblasts and SaOS-2-cells were seeded on multiple 6-well plates at a density of 5 × 10(5)cells in a 4-week cell culture. Four different concentrations (1, 5, 10, 20 μM) of each BP (ZOL, PAM, FOS) and pyrophosphate were used in this study.RESULTS: All BP decreased collagen production and lowered cell proliferation in-vitro. ZOL was the component with most inhibitory effect.CONCLUSION: The findings in this study suggest that ZOL, PAM and FOS generally diminish cell proliferation and collagen production of human gingival fibroblasts, osteoblasts and SaOS-2-cells. The present follow-up study shows that not only ZOL and PAM but also FOS have a strong inhibitory effect on collagen production and cell survival in-vitro.

KW - Alendronate

KW - Alkaline Phosphatase

KW - Bone Density Conservation Agents

KW - Cell Line, Tumor

KW - Cell Proliferation

KW - Cell Survival

KW - Cells, Cultured

KW - Chromatography, High Pressure Liquid

KW - Collagen Type I

KW - Coloring Agents

KW - Diphosphates

KW - Diphosphonates

KW - Electrophoresis, Polyacrylamide Gel

KW - Enzyme-Linked Immunosorbent Assay

KW - Fibroblasts

KW - Follow-Up Studies

KW - Gingiva

KW - Humans

KW - Imidazoles

KW - Osteoblasts

KW - Osteocalcin

KW - Osteosarcoma

KW - Tetrazolium Salts

KW - Thiazoles

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.jcms.2011.10.024

DO - 10.1016/j.jcms.2011.10.024

M3 - SCORING: Journal article

C2 - 22082730

VL - 40

SP - e229-35

JO - J CRANIO MAXILL SURG

JF - J CRANIO MAXILL SURG

SN - 1010-5182

IS - 8

ER -