The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells
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The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells. / Açil, Y; Möller, B; Niehoff, P; Rachko, K; Gassling, V; Wiltfang, J; Simon, M J K.
in: J CRANIO MAXILL SURG, Jahrgang 40, Nr. 8, 12.2012, S. e229-35.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The cytotoxic effects of three different bisphosphonates in-vitro on human gingival fibroblasts, osteoblasts and osteogenic sarcoma cells
AU - Açil, Y
AU - Möller, B
AU - Niehoff, P
AU - Rachko, K
AU - Gassling, V
AU - Wiltfang, J
AU - Simon, M J K
N1 - Copyright © 2011 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.
PY - 2012/12
Y1 - 2012/12
N2 - INTRODUCTION: Osteonecrosis of the jaw (ONJ) is an emerging condition in patients undergoing long-term administration of bisphosphonates (BP) for the treatment of osteoporosis and hypercalcaemia associated with malignancy, multiple myeloma, and metastatic breast and prostate cancers. This is a follow-up study, its purpose was to examine the effects in-vitro of intravenous zoledronic acid (ZOL) and pamidronate (PAM) and oral alendronate (FOS) on the human oral cavity using gingival fibroblasts and osteoblasts cells and, in addition, osteogenic sarcoma cells (SaOS-2-cells).MATERIALS AND METHODS: Human gingival fibroblasts, osteoblasts and SaOS-2-cells were seeded on multiple 6-well plates at a density of 5 × 10(5)cells in a 4-week cell culture. Four different concentrations (1, 5, 10, 20 μM) of each BP (ZOL, PAM, FOS) and pyrophosphate were used in this study.RESULTS: All BP decreased collagen production and lowered cell proliferation in-vitro. ZOL was the component with most inhibitory effect.CONCLUSION: The findings in this study suggest that ZOL, PAM and FOS generally diminish cell proliferation and collagen production of human gingival fibroblasts, osteoblasts and SaOS-2-cells. The present follow-up study shows that not only ZOL and PAM but also FOS have a strong inhibitory effect on collagen production and cell survival in-vitro.
AB - INTRODUCTION: Osteonecrosis of the jaw (ONJ) is an emerging condition in patients undergoing long-term administration of bisphosphonates (BP) for the treatment of osteoporosis and hypercalcaemia associated with malignancy, multiple myeloma, and metastatic breast and prostate cancers. This is a follow-up study, its purpose was to examine the effects in-vitro of intravenous zoledronic acid (ZOL) and pamidronate (PAM) and oral alendronate (FOS) on the human oral cavity using gingival fibroblasts and osteoblasts cells and, in addition, osteogenic sarcoma cells (SaOS-2-cells).MATERIALS AND METHODS: Human gingival fibroblasts, osteoblasts and SaOS-2-cells were seeded on multiple 6-well plates at a density of 5 × 10(5)cells in a 4-week cell culture. Four different concentrations (1, 5, 10, 20 μM) of each BP (ZOL, PAM, FOS) and pyrophosphate were used in this study.RESULTS: All BP decreased collagen production and lowered cell proliferation in-vitro. ZOL was the component with most inhibitory effect.CONCLUSION: The findings in this study suggest that ZOL, PAM and FOS generally diminish cell proliferation and collagen production of human gingival fibroblasts, osteoblasts and SaOS-2-cells. The present follow-up study shows that not only ZOL and PAM but also FOS have a strong inhibitory effect on collagen production and cell survival in-vitro.
KW - Alendronate
KW - Alkaline Phosphatase
KW - Bone Density Conservation Agents
KW - Cell Line, Tumor
KW - Cell Proliferation
KW - Cell Survival
KW - Cells, Cultured
KW - Chromatography, High Pressure Liquid
KW - Collagen Type I
KW - Coloring Agents
KW - Diphosphates
KW - Diphosphonates
KW - Electrophoresis, Polyacrylamide Gel
KW - Enzyme-Linked Immunosorbent Assay
KW - Fibroblasts
KW - Follow-Up Studies
KW - Gingiva
KW - Humans
KW - Imidazoles
KW - Osteoblasts
KW - Osteocalcin
KW - Osteosarcoma
KW - Tetrazolium Salts
KW - Thiazoles
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.jcms.2011.10.024
DO - 10.1016/j.jcms.2011.10.024
M3 - SCORING: Journal article
C2 - 22082730
VL - 40
SP - e229-35
JO - J CRANIO MAXILL SURG
JF - J CRANIO MAXILL SURG
SN - 1010-5182
IS - 8
ER -