The cytoplasmic tail of the T cell receptor zeta chain is required for signaling via CD26
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The cytoplasmic tail of the T cell receptor zeta chain is required for signaling via CD26. / Mittrücker, H W; Steeg, C; Malissen, B; Fleischer, B.
In: EUR J IMMUNOL, Vol. 25, No. 1, 01.01.1995, p. 295-7.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - The cytoplasmic tail of the T cell receptor zeta chain is required for signaling via CD26
AU - Mittrücker, H W
AU - Steeg, C
AU - Malissen, B
AU - Fleischer, B
PY - 1995/1/1
Y1 - 1995/1/1
N2 - The protease dipeptidylpeptidase IV (CD26) provides an alternative activation pathway for T lymphocytes and is involved in several aspects of T cell function. Activation via CD26 requires the expression of the T cell receptor (TcR)/CD3 complex. Here we have investigated the role of the TcR zeta chain for T cell activation via CD26. T cell hybridomas expressing TcR with various deletions in the CD3 zeta chain were transfected with a CD26 cDNA and the response of the transfected cells to anti-CD26 monoclonal antibodies was tested. Our data show that the zeta chain is essential and that at least one YXXL motif in the cytoplasmic tail of the zeta chain is required for CD26-mediated signaling. Other TcR components do not replace the zeta chain.
AB - The protease dipeptidylpeptidase IV (CD26) provides an alternative activation pathway for T lymphocytes and is involved in several aspects of T cell function. Activation via CD26 requires the expression of the T cell receptor (TcR)/CD3 complex. Here we have investigated the role of the TcR zeta chain for T cell activation via CD26. T cell hybridomas expressing TcR with various deletions in the CD3 zeta chain were transfected with a CD26 cDNA and the response of the transfected cells to anti-CD26 monoclonal antibodies was tested. Our data show that the zeta chain is essential and that at least one YXXL motif in the cytoplasmic tail of the zeta chain is required for CD26-mediated signaling. Other TcR components do not replace the zeta chain.
KW - Animals
KW - Dipeptidyl Peptidase 4
KW - Flow Cytometry
KW - Lymphocyte Activation
KW - Membrane Proteins
KW - Mice
KW - Mice, Inbred AKR
KW - Polymerase Chain Reaction
KW - Receptors, Antigen, T-Cell
KW - Signal Transduction
KW - Structure-Activity Relationship
KW - T-Lymphocytes
KW - Transfection
KW - Tumor Cells, Cultured
U2 - 10.1002/eji.1830250149
DO - 10.1002/eji.1830250149
M3 - SCORING: Journal article
C2 - 7843246
VL - 25
SP - 295
EP - 297
JO - EUR J IMMUNOL
JF - EUR J IMMUNOL
SN - 0014-2980
IS - 1
ER -