T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model
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T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model. / Schliffke, Simon; Carambia, Antonella; Akyüz, Nuray; Thiele, Benjamin; Herkel, Johannes; Binder, Mascha.
In: J NEUROIMMUNOL, Vol. 332, 15.07.2019, p. 49-56.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model
AU - Schliffke, Simon
AU - Carambia, Antonella
AU - Akyüz, Nuray
AU - Thiele, Benjamin
AU - Herkel, Johannes
AU - Binder, Mascha
N1 - Copyright © 2019 Elsevier B.V. All rights reserved.
PY - 2019/7/15
Y1 - 2019/7/15
N2 - The experimental autoimmune encephalomyelitis (EAE) model is indispensable for autoimmunity research, but model-specific T cell dynamics are sparsely studied. We used next-generation immunosequencing across lymphoid organs, blood and spinal cord in response to immunization with myelin basic protein (MBP) to study T cell repertoires and migration patterns. Surprisingly, most spinal cord T cells were unique to the individual animal despite the existence of shared MBP-specific clones, suggesting a previously underestimated T cell diversity. Almost complete emigration of pathogenic clones from blood to spinal cord indicates that blood is not a suitable compartment to study EAE-mediating T cells.
AB - The experimental autoimmune encephalomyelitis (EAE) model is indispensable for autoimmunity research, but model-specific T cell dynamics are sparsely studied. We used next-generation immunosequencing across lymphoid organs, blood and spinal cord in response to immunization with myelin basic protein (MBP) to study T cell repertoires and migration patterns. Surprisingly, most spinal cord T cells were unique to the individual animal despite the existence of shared MBP-specific clones, suggesting a previously underestimated T cell diversity. Almost complete emigration of pathogenic clones from blood to spinal cord indicates that blood is not a suitable compartment to study EAE-mediating T cells.
KW - Journal Article
U2 - 10.1016/j.jneuroim.2019.03.014
DO - 10.1016/j.jneuroim.2019.03.014
M3 - SCORING: Journal article
C2 - 30933850
VL - 332
SP - 49
EP - 56
JO - J NEUROIMMUNOL
JF - J NEUROIMMUNOL
SN - 0165-5728
ER -