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T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model

Standard

T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model. / Schliffke, Simon; Carambia, Antonella; Akyüz, Nuray; Thiele, Benjamin; Herkel, Johannes; Binder, Mascha.

in: J NEUROIMMUNOL, Jahrgang 332, 15.07.2019, S. 49-56.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Schliffke, S, Carambia, A, Akyüz, N, Thiele, B, Herkel, J & Binder, M 2019, 'T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model', J NEUROIMMUNOL, Jg. 332, S. 49-56. https://doi.org/10.1016/j.jneuroim.2019.03.014

APA

Schliffke, S., Carambia, A., Akyüz, N., Thiele, B., Herkel, J., & Binder, M. (2019). T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model. J NEUROIMMUNOL, 332, 49-56. https://doi.org/10.1016/j.jneuroim.2019.03.014

Vancouver

Schliffke S, Carambia A, Akyüz N, Thiele B, Herkel J, Binder M. T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model. J NEUROIMMUNOL. 2019 Jul 15;332:49-56. https://doi.org/10.1016/j.jneuroim.2019.03.014

Bibtex

@article{9c846c7113ee42408074a3fbc50267fe,
title = "T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model",
abstract = "The experimental autoimmune encephalomyelitis (EAE) model is indispensable for autoimmunity research, but model-specific T cell dynamics are sparsely studied. We used next-generation immunosequencing across lymphoid organs, blood and spinal cord in response to immunization with myelin basic protein (MBP) to study T cell repertoires and migration patterns. Surprisingly, most spinal cord T cells were unique to the individual animal despite the existence of shared MBP-specific clones, suggesting a previously underestimated T cell diversity. Almost complete emigration of pathogenic clones from blood to spinal cord indicates that blood is not a suitable compartment to study EAE-mediating T cells.",
keywords = "Journal Article",
author = "Simon Schliffke and Antonella Carambia and Nuray Aky{\"u}z and Benjamin Thiele and Johannes Herkel and Mascha Binder",
note = "Copyright {\textcopyright} 2019 Elsevier B.V. All rights reserved.",
year = "2019",
month = jul,
day = "15",
doi = "10.1016/j.jneuroim.2019.03.014",
language = "English",
volume = "332",
pages = "49--56",
journal = "J NEUROIMMUNOL",
issn = "0165-5728",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model

AU - Schliffke, Simon

AU - Carambia, Antonella

AU - Akyüz, Nuray

AU - Thiele, Benjamin

AU - Herkel, Johannes

AU - Binder, Mascha

N1 - Copyright © 2019 Elsevier B.V. All rights reserved.

PY - 2019/7/15

Y1 - 2019/7/15

N2 - The experimental autoimmune encephalomyelitis (EAE) model is indispensable for autoimmunity research, but model-specific T cell dynamics are sparsely studied. We used next-generation immunosequencing across lymphoid organs, blood and spinal cord in response to immunization with myelin basic protein (MBP) to study T cell repertoires and migration patterns. Surprisingly, most spinal cord T cells were unique to the individual animal despite the existence of shared MBP-specific clones, suggesting a previously underestimated T cell diversity. Almost complete emigration of pathogenic clones from blood to spinal cord indicates that blood is not a suitable compartment to study EAE-mediating T cells.

AB - The experimental autoimmune encephalomyelitis (EAE) model is indispensable for autoimmunity research, but model-specific T cell dynamics are sparsely studied. We used next-generation immunosequencing across lymphoid organs, blood and spinal cord in response to immunization with myelin basic protein (MBP) to study T cell repertoires and migration patterns. Surprisingly, most spinal cord T cells were unique to the individual animal despite the existence of shared MBP-specific clones, suggesting a previously underestimated T cell diversity. Almost complete emigration of pathogenic clones from blood to spinal cord indicates that blood is not a suitable compartment to study EAE-mediating T cells.

KW - Journal Article

U2 - 10.1016/j.jneuroim.2019.03.014

DO - 10.1016/j.jneuroim.2019.03.014

M3 - SCORING: Journal article

C2 - 30933850

VL - 332

SP - 49

EP - 56

JO - J NEUROIMMUNOL

JF - J NEUROIMMUNOL

SN - 0165-5728

ER -