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T-Cell Density at the Invasive Margin and Immune Phenotypes Predict Outcome in Vulvar Squamous Cell Cancer

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T-Cell Density at the Invasive Margin and Immune Phenotypes Predict Outcome in Vulvar Squamous Cell Cancer. / Burandt, Eike; Blessin, Niclas C; Rolschewski, Ann-Christin; Lutz, Florian; Mandelkow, Tim; Yang, Cheng; Bady, Elena; Reiswich, Viktor; Simon, Ronald; Sauter, Guido; Mahner, Sven; Gregorio, Nikolaus de; Klapdor, Rüdiger; Kalder, Matthias; Braicu, Elena I; Fürst, Sophie; Klar, Maximilian; Strauß, Hans-Georg; Prieske, Katharina; Wölber, Linn.

In: CANCERS, Vol. 14, No. 17, 4246, 31.08.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Burandt, E, Blessin, NC, Rolschewski, A-C, Lutz, F, Mandelkow, T, Yang, C, Bady, E, Reiswich, V, Simon, R, Sauter, G, Mahner, S, Gregorio, ND, Klapdor, R, Kalder, M, Braicu, EI, Fürst, S, Klar, M, Strauß, H-G, Prieske, K & Wölber, L 2022, 'T-Cell Density at the Invasive Margin and Immune Phenotypes Predict Outcome in Vulvar Squamous Cell Cancer', CANCERS, vol. 14, no. 17, 4246. https://doi.org/10.3390/cancers14174246

APA

Burandt, E., Blessin, N. C., Rolschewski, A-C., Lutz, F., Mandelkow, T., Yang, C., Bady, E., Reiswich, V., Simon, R., Sauter, G., Mahner, S., Gregorio, N. D., Klapdor, R., Kalder, M., Braicu, E. I., Fürst, S., Klar, M., Strauß, H-G., Prieske, K., & Wölber, L. (2022). T-Cell Density at the Invasive Margin and Immune Phenotypes Predict Outcome in Vulvar Squamous Cell Cancer. CANCERS, 14(17), [4246]. https://doi.org/10.3390/cancers14174246

Vancouver

Burandt E, Blessin NC, Rolschewski A-C, Lutz F, Mandelkow T, Yang C et al. T-Cell Density at the Invasive Margin and Immune Phenotypes Predict Outcome in Vulvar Squamous Cell Cancer. CANCERS. 2022 Aug 31;14(17). 4246. https://doi.org/10.3390/cancers14174246

Bibtex

@article{c1ddf850749141a2b68035b943edd658,
title = "T-Cell Density at the Invasive Margin and Immune Phenotypes Predict Outcome in Vulvar Squamous Cell Cancer",
abstract = "Background: Although quantification of tumor infiltrating lymphocytes (TILs) has become of increasing interest in immuno-oncology, only little is known about TILs infiltration in the tumor microenvironment and its predictive value in vulvar cancer. Methods: Immunohistochemistry and automated digital image analysis was applied to measure the densities of CD3+ (DAKO, #IR503) and CD8+ (DAKO, #IR623) TILs at the invasive margin and in the center of 530 vulvar squamous cell cancers. Results: An elevated density of CD3+ T-cell at the invasive margin was significantly associated with low tumor stage (p = 0.0012) and prolonged survival (overall survival [OS] p = 0.0027, progression free survival [PFS] p = 0.024) and was independent from tumor stage, nodal stage, grade, and HPV-status in multivariate analysis (p < 0.05). The prognostic impact of CD3+ cells in the center of the tumor was weaker compared to the invasive margin (OS p = 0.046, PFS p = 0.031) and lacking for CD8+ T-cell densities at any location (p ≥ 0.14 each). Unsupervised clustering of CD3+ and CD8+ T-cell densities identified three major subgroups corresponding to the immune desert (137 patients), immune excluded (220 patients) and immune inflamed phenotypes (133 patients). Survival analysis revealed a particular poor prognosis for the immune desert phenotype for OS (p = 0.0071) and PFS (p = 0.0027). Conclusion: Our data demonstrate a high prognostic value of CD3+ T-cells at the invasive margin and immune phenotypes in vulvar squamous cell cancer.",
author = "Eike Burandt and Blessin, {Niclas C} and Ann-Christin Rolschewski and Florian Lutz and Tim Mandelkow and Cheng Yang and Elena Bady and Viktor Reiswich and Ronald Simon and Guido Sauter and Sven Mahner and Gregorio, {Nikolaus de} and R{\"u}diger Klapdor and Matthias Kalder and Braicu, {Elena I} and Sophie F{\"u}rst and Maximilian Klar and Hans-Georg Strau{\ss} and Katharina Prieske and Linn W{\"o}lber",
year = "2022",
month = aug,
day = "31",
doi = "10.3390/cancers14174246",
language = "English",
volume = "14",
journal = "CANCERS",
issn = "2072-6694",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "17",

}

RIS

TY - JOUR

T1 - T-Cell Density at the Invasive Margin and Immune Phenotypes Predict Outcome in Vulvar Squamous Cell Cancer

AU - Burandt, Eike

AU - Blessin, Niclas C

AU - Rolschewski, Ann-Christin

AU - Lutz, Florian

AU - Mandelkow, Tim

AU - Yang, Cheng

AU - Bady, Elena

AU - Reiswich, Viktor

AU - Simon, Ronald

AU - Sauter, Guido

AU - Mahner, Sven

AU - Gregorio, Nikolaus de

AU - Klapdor, Rüdiger

AU - Kalder, Matthias

AU - Braicu, Elena I

AU - Fürst, Sophie

AU - Klar, Maximilian

AU - Strauß, Hans-Georg

AU - Prieske, Katharina

AU - Wölber, Linn

PY - 2022/8/31

Y1 - 2022/8/31

N2 - Background: Although quantification of tumor infiltrating lymphocytes (TILs) has become of increasing interest in immuno-oncology, only little is known about TILs infiltration in the tumor microenvironment and its predictive value in vulvar cancer. Methods: Immunohistochemistry and automated digital image analysis was applied to measure the densities of CD3+ (DAKO, #IR503) and CD8+ (DAKO, #IR623) TILs at the invasive margin and in the center of 530 vulvar squamous cell cancers. Results: An elevated density of CD3+ T-cell at the invasive margin was significantly associated with low tumor stage (p = 0.0012) and prolonged survival (overall survival [OS] p = 0.0027, progression free survival [PFS] p = 0.024) and was independent from tumor stage, nodal stage, grade, and HPV-status in multivariate analysis (p < 0.05). The prognostic impact of CD3+ cells in the center of the tumor was weaker compared to the invasive margin (OS p = 0.046, PFS p = 0.031) and lacking for CD8+ T-cell densities at any location (p ≥ 0.14 each). Unsupervised clustering of CD3+ and CD8+ T-cell densities identified three major subgroups corresponding to the immune desert (137 patients), immune excluded (220 patients) and immune inflamed phenotypes (133 patients). Survival analysis revealed a particular poor prognosis for the immune desert phenotype for OS (p = 0.0071) and PFS (p = 0.0027). Conclusion: Our data demonstrate a high prognostic value of CD3+ T-cells at the invasive margin and immune phenotypes in vulvar squamous cell cancer.

AB - Background: Although quantification of tumor infiltrating lymphocytes (TILs) has become of increasing interest in immuno-oncology, only little is known about TILs infiltration in the tumor microenvironment and its predictive value in vulvar cancer. Methods: Immunohistochemistry and automated digital image analysis was applied to measure the densities of CD3+ (DAKO, #IR503) and CD8+ (DAKO, #IR623) TILs at the invasive margin and in the center of 530 vulvar squamous cell cancers. Results: An elevated density of CD3+ T-cell at the invasive margin was significantly associated with low tumor stage (p = 0.0012) and prolonged survival (overall survival [OS] p = 0.0027, progression free survival [PFS] p = 0.024) and was independent from tumor stage, nodal stage, grade, and HPV-status in multivariate analysis (p < 0.05). The prognostic impact of CD3+ cells in the center of the tumor was weaker compared to the invasive margin (OS p = 0.046, PFS p = 0.031) and lacking for CD8+ T-cell densities at any location (p ≥ 0.14 each). Unsupervised clustering of CD3+ and CD8+ T-cell densities identified three major subgroups corresponding to the immune desert (137 patients), immune excluded (220 patients) and immune inflamed phenotypes (133 patients). Survival analysis revealed a particular poor prognosis for the immune desert phenotype for OS (p = 0.0071) and PFS (p = 0.0027). Conclusion: Our data demonstrate a high prognostic value of CD3+ T-cells at the invasive margin and immune phenotypes in vulvar squamous cell cancer.

U2 - 10.3390/cancers14174246

DO - 10.3390/cancers14174246

M3 - SCORING: Journal article

C2 - 36077784

VL - 14

JO - CANCERS

JF - CANCERS

SN - 2072-6694

IS - 17

M1 - 4246

ER -