T-Cell Density at the Invasive Margin and Immune Phenotypes Predict Outcome in Vulvar Squamous Cell Cancer
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T-Cell Density at the Invasive Margin and Immune Phenotypes Predict Outcome in Vulvar Squamous Cell Cancer. / Burandt, Eike; Blessin, Niclas C; Rolschewski, Ann-Christin; Lutz, Florian; Mandelkow, Tim; Yang, Cheng; Bady, Elena; Reiswich, Viktor; Simon, Ronald; Sauter, Guido; Mahner, Sven; Gregorio, Nikolaus de; Klapdor, Rüdiger; Kalder, Matthias; Braicu, Elena I; Fürst, Sophie; Klar, Maximilian; Strauß, Hans-Georg; Prieske, Katharina; Wölber, Linn.
in: CANCERS, Jahrgang 14, Nr. 17, 4246, 31.08.2022.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - T-Cell Density at the Invasive Margin and Immune Phenotypes Predict Outcome in Vulvar Squamous Cell Cancer
AU - Burandt, Eike
AU - Blessin, Niclas C
AU - Rolschewski, Ann-Christin
AU - Lutz, Florian
AU - Mandelkow, Tim
AU - Yang, Cheng
AU - Bady, Elena
AU - Reiswich, Viktor
AU - Simon, Ronald
AU - Sauter, Guido
AU - Mahner, Sven
AU - Gregorio, Nikolaus de
AU - Klapdor, Rüdiger
AU - Kalder, Matthias
AU - Braicu, Elena I
AU - Fürst, Sophie
AU - Klar, Maximilian
AU - Strauß, Hans-Georg
AU - Prieske, Katharina
AU - Wölber, Linn
PY - 2022/8/31
Y1 - 2022/8/31
N2 - Background: Although quantification of tumor infiltrating lymphocytes (TILs) has become of increasing interest in immuno-oncology, only little is known about TILs infiltration in the tumor microenvironment and its predictive value in vulvar cancer. Methods: Immunohistochemistry and automated digital image analysis was applied to measure the densities of CD3+ (DAKO, #IR503) and CD8+ (DAKO, #IR623) TILs at the invasive margin and in the center of 530 vulvar squamous cell cancers. Results: An elevated density of CD3+ T-cell at the invasive margin was significantly associated with low tumor stage (p = 0.0012) and prolonged survival (overall survival [OS] p = 0.0027, progression free survival [PFS] p = 0.024) and was independent from tumor stage, nodal stage, grade, and HPV-status in multivariate analysis (p < 0.05). The prognostic impact of CD3+ cells in the center of the tumor was weaker compared to the invasive margin (OS p = 0.046, PFS p = 0.031) and lacking for CD8+ T-cell densities at any location (p ≥ 0.14 each). Unsupervised clustering of CD3+ and CD8+ T-cell densities identified three major subgroups corresponding to the immune desert (137 patients), immune excluded (220 patients) and immune inflamed phenotypes (133 patients). Survival analysis revealed a particular poor prognosis for the immune desert phenotype for OS (p = 0.0071) and PFS (p = 0.0027). Conclusion: Our data demonstrate a high prognostic value of CD3+ T-cells at the invasive margin and immune phenotypes in vulvar squamous cell cancer.
AB - Background: Although quantification of tumor infiltrating lymphocytes (TILs) has become of increasing interest in immuno-oncology, only little is known about TILs infiltration in the tumor microenvironment and its predictive value in vulvar cancer. Methods: Immunohistochemistry and automated digital image analysis was applied to measure the densities of CD3+ (DAKO, #IR503) and CD8+ (DAKO, #IR623) TILs at the invasive margin and in the center of 530 vulvar squamous cell cancers. Results: An elevated density of CD3+ T-cell at the invasive margin was significantly associated with low tumor stage (p = 0.0012) and prolonged survival (overall survival [OS] p = 0.0027, progression free survival [PFS] p = 0.024) and was independent from tumor stage, nodal stage, grade, and HPV-status in multivariate analysis (p < 0.05). The prognostic impact of CD3+ cells in the center of the tumor was weaker compared to the invasive margin (OS p = 0.046, PFS p = 0.031) and lacking for CD8+ T-cell densities at any location (p ≥ 0.14 each). Unsupervised clustering of CD3+ and CD8+ T-cell densities identified three major subgroups corresponding to the immune desert (137 patients), immune excluded (220 patients) and immune inflamed phenotypes (133 patients). Survival analysis revealed a particular poor prognosis for the immune desert phenotype for OS (p = 0.0071) and PFS (p = 0.0027). Conclusion: Our data demonstrate a high prognostic value of CD3+ T-cells at the invasive margin and immune phenotypes in vulvar squamous cell cancer.
U2 - 10.3390/cancers14174246
DO - 10.3390/cancers14174246
M3 - SCORING: Journal article
C2 - 36077784
VL - 14
JO - CANCERS
JF - CANCERS
SN - 2072-6694
IS - 17
M1 - 4246
ER -