Taurocholate-induced pancreatitis
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Taurocholate-induced pancreatitis : a model of severe necrotizing pancreatitis in mice. / Wittel, Uwe A; Wiech, Thorsten; Chakraborty, Subhankar; Boss, Babette; Lauch, Robert; Batra, Surinder K; Hopt, Ulrich T.
In: PANCREAS, Vol. 36, No. 2, 01.03.2008, p. e9-21.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Taurocholate-induced pancreatitis
T2 - a model of severe necrotizing pancreatitis in mice
AU - Wittel, Uwe A
AU - Wiech, Thorsten
AU - Chakraborty, Subhankar
AU - Boss, Babette
AU - Lauch, Robert
AU - Batra, Surinder K
AU - Hopt, Ulrich T
PY - 2008/3/1
Y1 - 2008/3/1
N2 - OBJECTIVES: The outcome from acute pancreatitis depends on the severity of systemic complications. To be able to investigate mechanisms underlying the development of these systemic complications in acute pancreatitis in both wild-type and genetically engineered animal models, a mouse model of severe necrotizing pancreatitis was developed and characterized.METHODS: Pancreatitis was induced by retrograde infusion of sodium taurocholate into the common bile duct in mice. After determining the optimum volume and concentration of taurocholate, the pancreatic damage and systemic inflammatory response were compared with those in cerulein-induced pancreatitis.RESULTS: Pancreatic damage was higher in taurocholate pancreatitis than hyperstimulation-induced pancreatitis (24 hours: cerulein, 5.8 +/- 0.2 points; taurocholate, 14.8 +/- 0.8 points; P < 0.001) and mortality reached up to 60% within the first 24 hours after taurocholate administration. Pulmonary damage was detected, as measured by an increase in albumin in bronchoalveolar lavage fluid only in taurocholate-induced pancreatitis (12 hours: cerulein, 97.1 +/- 22.83 mg/g of protein; taurocholate, 234.0 +/- 32.7 mg/g of protein; P < 0.001). Furthermore, plasma interleukin 6 concentration was significantly elevated in mice with taurocholate-induced pancreatitis (12 hours: cerulein, 2.6 +/- 6.1 pg/mL; taurocholate, 2168.8 +/- 941.7 microg/mL; P < 0.001) as compared with all other groups.CONCLUSIONS: Taurocholate pancreatitis is a reliable model for severe necrotizing pancreatitis in mice with significantly greater pancreatic damage and systemic inflammatory response in comparison with cerulein-induced pancreatitis.
AB - OBJECTIVES: The outcome from acute pancreatitis depends on the severity of systemic complications. To be able to investigate mechanisms underlying the development of these systemic complications in acute pancreatitis in both wild-type and genetically engineered animal models, a mouse model of severe necrotizing pancreatitis was developed and characterized.METHODS: Pancreatitis was induced by retrograde infusion of sodium taurocholate into the common bile duct in mice. After determining the optimum volume and concentration of taurocholate, the pancreatic damage and systemic inflammatory response were compared with those in cerulein-induced pancreatitis.RESULTS: Pancreatic damage was higher in taurocholate pancreatitis than hyperstimulation-induced pancreatitis (24 hours: cerulein, 5.8 +/- 0.2 points; taurocholate, 14.8 +/- 0.8 points; P < 0.001) and mortality reached up to 60% within the first 24 hours after taurocholate administration. Pulmonary damage was detected, as measured by an increase in albumin in bronchoalveolar lavage fluid only in taurocholate-induced pancreatitis (12 hours: cerulein, 97.1 +/- 22.83 mg/g of protein; taurocholate, 234.0 +/- 32.7 mg/g of protein; P < 0.001). Furthermore, plasma interleukin 6 concentration was significantly elevated in mice with taurocholate-induced pancreatitis (12 hours: cerulein, 2.6 +/- 6.1 pg/mL; taurocholate, 2168.8 +/- 941.7 microg/mL; P < 0.001) as compared with all other groups.CONCLUSIONS: Taurocholate pancreatitis is a reliable model for severe necrotizing pancreatitis in mice with significantly greater pancreatic damage and systemic inflammatory response in comparison with cerulein-induced pancreatitis.
KW - Albumins
KW - Amylases
KW - Animals
KW - Bronchoalveolar Lavage Fluid
KW - Ceruletide
KW - Disease Models, Animal
KW - Dose-Response Relationship, Drug
KW - Feasibility Studies
KW - Inflammation
KW - Injections
KW - Interleukin-6
KW - Lipase
KW - Lung Diseases
KW - Male
KW - Mice
KW - Mice, Inbred BALB C
KW - Pancreas
KW - Pancreatitis, Acute Necrotizing
KW - Reproducibility of Results
KW - Severity of Illness Index
KW - Taurocholic Acid
KW - Time Factors
U2 - 10.1097/MPA.0b013e3181575103
DO - 10.1097/MPA.0b013e3181575103
M3 - SCORING: Journal article
C2 - 18376298
VL - 36
SP - e9-21
JO - PANCREAS
JF - PANCREAS
SN - 0885-3177
IS - 2
ER -