Taurocholate-induced pancreatitis

TY - JOUR

T1 - Taurocholate-induced pancreatitis

T2 - a model of severe necrotizing pancreatitis in mice

AU - Wittel, Uwe A

AU - Wiech, Thorsten

AU - Chakraborty, Subhankar

AU - Boss, Babette

AU - Lauch, Robert

AU - Batra, Surinder K

AU - Hopt, Ulrich T

PY - 2008/3/1

Y1 - 2008/3/1

N2 - OBJECTIVES: The outcome from acute pancreatitis depends on the severity of systemic complications. To be able to investigate mechanisms underlying the development of these systemic complications in acute pancreatitis in both wild-type and genetically engineered animal models, a mouse model of severe necrotizing pancreatitis was developed and characterized.METHODS: Pancreatitis was induced by retrograde infusion of sodium taurocholate into the common bile duct in mice. After determining the optimum volume and concentration of taurocholate, the pancreatic damage and systemic inflammatory response were compared with those in cerulein-induced pancreatitis.RESULTS: Pancreatic damage was higher in taurocholate pancreatitis than hyperstimulation-induced pancreatitis (24 hours: cerulein, 5.8 +/- 0.2 points; taurocholate, 14.8 +/- 0.8 points; P < 0.001) and mortality reached up to 60% within the first 24 hours after taurocholate administration. Pulmonary damage was detected, as measured by an increase in albumin in bronchoalveolar lavage fluid only in taurocholate-induced pancreatitis (12 hours: cerulein, 97.1 +/- 22.83 mg/g of protein; taurocholate, 234.0 +/- 32.7 mg/g of protein; P < 0.001). Furthermore, plasma interleukin 6 concentration was significantly elevated in mice with taurocholate-induced pancreatitis (12 hours: cerulein, 2.6 +/- 6.1 pg/mL; taurocholate, 2168.8 +/- 941.7 microg/mL; P < 0.001) as compared with all other groups.CONCLUSIONS: Taurocholate pancreatitis is a reliable model for severe necrotizing pancreatitis in mice with significantly greater pancreatic damage and systemic inflammatory response in comparison with cerulein-induced pancreatitis.

AB - OBJECTIVES: The outcome from acute pancreatitis depends on the severity of systemic complications. To be able to investigate mechanisms underlying the development of these systemic complications in acute pancreatitis in both wild-type and genetically engineered animal models, a mouse model of severe necrotizing pancreatitis was developed and characterized.METHODS: Pancreatitis was induced by retrograde infusion of sodium taurocholate into the common bile duct in mice. After determining the optimum volume and concentration of taurocholate, the pancreatic damage and systemic inflammatory response were compared with those in cerulein-induced pancreatitis.RESULTS: Pancreatic damage was higher in taurocholate pancreatitis than hyperstimulation-induced pancreatitis (24 hours: cerulein, 5.8 +/- 0.2 points; taurocholate, 14.8 +/- 0.8 points; P < 0.001) and mortality reached up to 60% within the first 24 hours after taurocholate administration. Pulmonary damage was detected, as measured by an increase in albumin in bronchoalveolar lavage fluid only in taurocholate-induced pancreatitis (12 hours: cerulein, 97.1 +/- 22.83 mg/g of protein; taurocholate, 234.0 +/- 32.7 mg/g of protein; P < 0.001). Furthermore, plasma interleukin 6 concentration was significantly elevated in mice with taurocholate-induced pancreatitis (12 hours: cerulein, 2.6 +/- 6.1 pg/mL; taurocholate, 2168.8 +/- 941.7 microg/mL; P < 0.001) as compared with all other groups.CONCLUSIONS: Taurocholate pancreatitis is a reliable model for severe necrotizing pancreatitis in mice with significantly greater pancreatic damage and systemic inflammatory response in comparison with cerulein-induced pancreatitis.

KW - Albumins

KW - Amylases

KW - Animals

KW - Bronchoalveolar Lavage Fluid

KW - Ceruletide

KW - Disease Models, Animal

KW - Dose-Response Relationship, Drug

KW - Feasibility Studies

KW - Inflammation

KW - Injections

KW - Interleukin-6

KW - Lipase

KW - Lung Diseases

KW - Male

KW - Mice

KW - Mice, Inbred BALB C

KW - Pancreas

KW - Pancreatitis, Acute Necrotizing

KW - Reproducibility of Results

KW - Severity of Illness Index

KW - Taurocholic Acid

KW - Time Factors

U2 - 10.1097/MPA.0b013e3181575103

DO - 10.1097/MPA.0b013e3181575103

M3 - SCORING: Journal article

C2 - 18376298

VL - 36

SP - e9-21

JO - PANCREAS

JF - PANCREAS

SN - 0885-3177

IS - 2

ER -