Tailor-made quantum dot and iron oxide based contrast agents for in vitro and in vivo tumor imaging.
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Tailor-made quantum dot and iron oxide based contrast agents for in vitro and in vivo tumor imaging. / Pöselt, Elmar; Schmidtke, Christian; Fischer, Steffen; Peldschus, Kersten; Salamon, Johannes Martin; Kloust, Hauke; Tran, Huong; Pietsch, Andrea; Heine, Markus; Adam, Gerhard; Schumacher, Udo; Wagener, Christoph; Förster, Stephan; Weller, Horst.
In: ACS NANO, Vol. 6, No. 4, 4, 2012, p. 3346-3355.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Tailor-made quantum dot and iron oxide based contrast agents for in vitro and in vivo tumor imaging.
AU - Pöselt, Elmar
AU - Schmidtke, Christian
AU - Fischer, Steffen
AU - Peldschus, Kersten
AU - Salamon, Johannes Martin
AU - Kloust, Hauke
AU - Tran, Huong
AU - Pietsch, Andrea
AU - Heine, Markus
AU - Adam, Gerhard
AU - Schumacher, Udo
AU - Wagener, Christoph
AU - Förster, Stephan
AU - Weller, Horst
PY - 2012
Y1 - 2012
N2 - The biofunctionalization of CdSe/CdS/ZnS quantum dots and Fe(3)O(4) nanocrystals using a novel ligand system based on polyisoprene-block-poly(ethylene oxide) ligands is described. The synthesis includes a partial ligand exchange of the hydrophobic nanocrystals with amino-functionalized polyisoprene ligands, followed by seeded micelle formation of the diblock-copolymers in water. The resulting water-soluble quantum dots showed fluorescence quantum efficiencies in the 40 to 50% range and extraordinary fluorescence stability in the biological environment after cross-linking of the polyisoprene moiety of the ligand shell. No toxicity was detected by water-soluble tetrazolium (WST8) and lactate dehydrogenase (LDH) assays, even at very high nanoparticle concentrations, and almost no nonspecific cell adhesion was detected. The ligand shell was further coupled to the antigen-related cell adhesion molecule (CEACAM) specific monoclonal antibody T84.1. The so-conjugated Fe(3)O(4) nanocrystals allowed in vitro and in vivo tumor targeting by magnetic resonance imaging.
AB - The biofunctionalization of CdSe/CdS/ZnS quantum dots and Fe(3)O(4) nanocrystals using a novel ligand system based on polyisoprene-block-poly(ethylene oxide) ligands is described. The synthesis includes a partial ligand exchange of the hydrophobic nanocrystals with amino-functionalized polyisoprene ligands, followed by seeded micelle formation of the diblock-copolymers in water. The resulting water-soluble quantum dots showed fluorescence quantum efficiencies in the 40 to 50% range and extraordinary fluorescence stability in the biological environment after cross-linking of the polyisoprene moiety of the ligand shell. No toxicity was detected by water-soluble tetrazolium (WST8) and lactate dehydrogenase (LDH) assays, even at very high nanoparticle concentrations, and almost no nonspecific cell adhesion was detected. The ligand shell was further coupled to the antigen-related cell adhesion molecule (CEACAM) specific monoclonal antibody T84.1. The so-conjugated Fe(3)O(4) nanocrystals allowed in vitro and in vivo tumor targeting by magnetic resonance imaging.
M3 - SCORING: Journal article
VL - 6
SP - 3346
EP - 3355
JO - ACS NANO
JF - ACS NANO
SN - 1936-0851
IS - 4
M1 - 4
ER -