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T3-mediated gene expression is independent of PGC-1alpha

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T3-mediated gene expression is independent of PGC-1alpha. / Wulf, Anne; Harneit, Angelika; Weitzel, Joachim M.

In: MOL CELL ENDOCRINOL, Vol. 270, No. 1-2, 30.05.2007, p. 57-63.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wulf, A, Harneit, A & Weitzel, JM 2007, 'T3-mediated gene expression is independent of PGC-1alpha', MOL CELL ENDOCRINOL, vol. 270, no. 1-2, pp. 57-63. https://doi.org/10.1016/j.mce.2007.02.008

APA

Wulf, A., Harneit, A., & Weitzel, J. M. (2007). T3-mediated gene expression is independent of PGC-1alpha. MOL CELL ENDOCRINOL, 270(1-2), 57-63. https://doi.org/10.1016/j.mce.2007.02.008

Vancouver

Wulf A, Harneit A, Weitzel JM. T3-mediated gene expression is independent of PGC-1alpha. MOL CELL ENDOCRINOL. 2007 May 30;270(1-2):57-63. https://doi.org/10.1016/j.mce.2007.02.008

Bibtex

@article{742531c88bee4e7e93ff33bf8a94598c,
title = "T3-mediated gene expression is independent of PGC-1alpha",
abstract = "Thyroid hormone (T3) has a profound influence on normal development, differentiation and metabolism, processes which are known to be regulated by the transcriptional coactivator PGC-1alpha (peroxisome proliferator-activated receptor gamma coactivator-1alpha). Since T3 rapidly induces PGC-1alpha expression, we investigated whether reduced PGC-1alpha levels lead to alterations in T3-mediated gene expression patterns. Using RNA interference, we reduced PGC-1alpha mRNA to approximately 10% of its initial concentration in rat pituitary GC cells. Knock-down of PGC-1alpha is accompanied by diminished protein concentration and decreased expression level of PGC-1alpha target genes, among them key enzymes involved in gluconeogenesis, mitochondrial biogenesis and fatty acid oxidation. PGC-1alpha, PGC-1beta and NRF-1 mRNA molecules were rapidly degraded with a half-life time of approximately 90min, but this was independent of T3 stimulation. Expression of T3-target genes was not changed upon knock-down of PGC-1alpha. Our data indicate that complex T3-mediated gene expression patterns are maintained independently of PGC-1alpha activation.",
keywords = "Animals, Gene Expression Regulation, Half-Life, Liver, Male, Pituitary Gland, RNA-Binding Proteins, Rats, Transcription Factors, Triiodothyronine",
author = "Anne Wulf and Angelika Harneit and Weitzel, {Joachim M}",
year = "2007",
month = may,
day = "30",
doi = "10.1016/j.mce.2007.02.008",
language = "English",
volume = "270",
pages = "57--63",
journal = "MOL CELL ENDOCRINOL",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

RIS

TY - JOUR

T1 - T3-mediated gene expression is independent of PGC-1alpha

AU - Wulf, Anne

AU - Harneit, Angelika

AU - Weitzel, Joachim M

PY - 2007/5/30

Y1 - 2007/5/30

N2 - Thyroid hormone (T3) has a profound influence on normal development, differentiation and metabolism, processes which are known to be regulated by the transcriptional coactivator PGC-1alpha (peroxisome proliferator-activated receptor gamma coactivator-1alpha). Since T3 rapidly induces PGC-1alpha expression, we investigated whether reduced PGC-1alpha levels lead to alterations in T3-mediated gene expression patterns. Using RNA interference, we reduced PGC-1alpha mRNA to approximately 10% of its initial concentration in rat pituitary GC cells. Knock-down of PGC-1alpha is accompanied by diminished protein concentration and decreased expression level of PGC-1alpha target genes, among them key enzymes involved in gluconeogenesis, mitochondrial biogenesis and fatty acid oxidation. PGC-1alpha, PGC-1beta and NRF-1 mRNA molecules were rapidly degraded with a half-life time of approximately 90min, but this was independent of T3 stimulation. Expression of T3-target genes was not changed upon knock-down of PGC-1alpha. Our data indicate that complex T3-mediated gene expression patterns are maintained independently of PGC-1alpha activation.

AB - Thyroid hormone (T3) has a profound influence on normal development, differentiation and metabolism, processes which are known to be regulated by the transcriptional coactivator PGC-1alpha (peroxisome proliferator-activated receptor gamma coactivator-1alpha). Since T3 rapidly induces PGC-1alpha expression, we investigated whether reduced PGC-1alpha levels lead to alterations in T3-mediated gene expression patterns. Using RNA interference, we reduced PGC-1alpha mRNA to approximately 10% of its initial concentration in rat pituitary GC cells. Knock-down of PGC-1alpha is accompanied by diminished protein concentration and decreased expression level of PGC-1alpha target genes, among them key enzymes involved in gluconeogenesis, mitochondrial biogenesis and fatty acid oxidation. PGC-1alpha, PGC-1beta and NRF-1 mRNA molecules were rapidly degraded with a half-life time of approximately 90min, but this was independent of T3 stimulation. Expression of T3-target genes was not changed upon knock-down of PGC-1alpha. Our data indicate that complex T3-mediated gene expression patterns are maintained independently of PGC-1alpha activation.

KW - Animals

KW - Gene Expression Regulation

KW - Half-Life

KW - Liver

KW - Male

KW - Pituitary Gland

KW - RNA-Binding Proteins

KW - Rats

KW - Transcription Factors

KW - Triiodothyronine

U2 - 10.1016/j.mce.2007.02.008

DO - 10.1016/j.mce.2007.02.008

M3 - SCORING: Journal article

C2 - 17382463

VL - 270

SP - 57

EP - 63

JO - MOL CELL ENDOCRINOL

JF - MOL CELL ENDOCRINOL

SN - 0303-7207

IS - 1-2

ER -