T3-mediated gene expression is independent of PGC-1alpha
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T3-mediated gene expression is independent of PGC-1alpha. / Wulf, Anne; Harneit, Angelika; Weitzel, Joachim M.
in: MOL CELL ENDOCRINOL, Jahrgang 270, Nr. 1-2, 30.05.2007, S. 57-63.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - T3-mediated gene expression is independent of PGC-1alpha
AU - Wulf, Anne
AU - Harneit, Angelika
AU - Weitzel, Joachim M
PY - 2007/5/30
Y1 - 2007/5/30
N2 - Thyroid hormone (T3) has a profound influence on normal development, differentiation and metabolism, processes which are known to be regulated by the transcriptional coactivator PGC-1alpha (peroxisome proliferator-activated receptor gamma coactivator-1alpha). Since T3 rapidly induces PGC-1alpha expression, we investigated whether reduced PGC-1alpha levels lead to alterations in T3-mediated gene expression patterns. Using RNA interference, we reduced PGC-1alpha mRNA to approximately 10% of its initial concentration in rat pituitary GC cells. Knock-down of PGC-1alpha is accompanied by diminished protein concentration and decreased expression level of PGC-1alpha target genes, among them key enzymes involved in gluconeogenesis, mitochondrial biogenesis and fatty acid oxidation. PGC-1alpha, PGC-1beta and NRF-1 mRNA molecules were rapidly degraded with a half-life time of approximately 90min, but this was independent of T3 stimulation. Expression of T3-target genes was not changed upon knock-down of PGC-1alpha. Our data indicate that complex T3-mediated gene expression patterns are maintained independently of PGC-1alpha activation.
AB - Thyroid hormone (T3) has a profound influence on normal development, differentiation and metabolism, processes which are known to be regulated by the transcriptional coactivator PGC-1alpha (peroxisome proliferator-activated receptor gamma coactivator-1alpha). Since T3 rapidly induces PGC-1alpha expression, we investigated whether reduced PGC-1alpha levels lead to alterations in T3-mediated gene expression patterns. Using RNA interference, we reduced PGC-1alpha mRNA to approximately 10% of its initial concentration in rat pituitary GC cells. Knock-down of PGC-1alpha is accompanied by diminished protein concentration and decreased expression level of PGC-1alpha target genes, among them key enzymes involved in gluconeogenesis, mitochondrial biogenesis and fatty acid oxidation. PGC-1alpha, PGC-1beta and NRF-1 mRNA molecules were rapidly degraded with a half-life time of approximately 90min, but this was independent of T3 stimulation. Expression of T3-target genes was not changed upon knock-down of PGC-1alpha. Our data indicate that complex T3-mediated gene expression patterns are maintained independently of PGC-1alpha activation.
KW - Animals
KW - Gene Expression Regulation
KW - Half-Life
KW - Liver
KW - Male
KW - Pituitary Gland
KW - RNA-Binding Proteins
KW - Rats
KW - Transcription Factors
KW - Triiodothyronine
U2 - 10.1016/j.mce.2007.02.008
DO - 10.1016/j.mce.2007.02.008
M3 - SCORING: Journal article
C2 - 17382463
VL - 270
SP - 57
EP - 63
JO - MOL CELL ENDOCRINOL
JF - MOL CELL ENDOCRINOL
SN - 0303-7207
IS - 1-2
ER -