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SWATH Mass Spectrometry for Proteomics of Non-Depleted Plasma

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SWATH Mass Spectrometry for Proteomics of Non-Depleted Plasma. / Krisp, Christoph; Molloy, Mark P.

In: Methods Mol Biol, Vol. 1619, 2017, p. 373-383.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Krisp, C & Molloy, MP 2017, 'SWATH Mass Spectrometry for Proteomics of Non-Depleted Plasma', Methods Mol Biol, vol. 1619, pp. 373-383. https://doi.org/10.1007/978-1-4939-7057-5_25

APA

Krisp, C., & Molloy, M. P. (2017). SWATH Mass Spectrometry for Proteomics of Non-Depleted Plasma. Methods Mol Biol, 1619, 373-383. https://doi.org/10.1007/978-1-4939-7057-5_25

Vancouver

Krisp C, Molloy MP. SWATH Mass Spectrometry for Proteomics of Non-Depleted Plasma. Methods Mol Biol. 2017;1619:373-383. https://doi.org/10.1007/978-1-4939-7057-5_25

Bibtex

@article{e2743da38026431a9d7152d99a665491,
title = "SWATH Mass Spectrometry for Proteomics of Non-Depleted Plasma",
abstract = "The limitations commonly observed in data-dependent acquisition (DDA) mass spectrometric investigation of non-depleted human plasma are mainly due to the large dynamic concentration range of protein expression. Less abundant proteins are usually masked by highly abundant proteins and are therefore difficult to reliably detect. Sequential window acquisition of all theoretical fragment-ion spectra (SWATH) mass spectrometry (MS), as a representative of data-independent acquisition (DIA) approaches, provides an opportunity to improve plasma-based biomarker discovery studies because this approach does not rely on precursor intensity for fragmentation selection but rather analyzes all precursors in specified mass ranges. Here, we describe a workflow for SWATH-MS-based analysis of non-depleted plasma including sample preparation, data acquisition, and statistical analysis.",
keywords = "Journal Article",
author = "Christoph Krisp and Molloy, {Mark P}",
year = "2017",
doi = "10.1007/978-1-4939-7057-5_25",
language = "English",
volume = "1619",
pages = "373--383",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - SWATH Mass Spectrometry for Proteomics of Non-Depleted Plasma

AU - Krisp, Christoph

AU - Molloy, Mark P

PY - 2017

Y1 - 2017

N2 - The limitations commonly observed in data-dependent acquisition (DDA) mass spectrometric investigation of non-depleted human plasma are mainly due to the large dynamic concentration range of protein expression. Less abundant proteins are usually masked by highly abundant proteins and are therefore difficult to reliably detect. Sequential window acquisition of all theoretical fragment-ion spectra (SWATH) mass spectrometry (MS), as a representative of data-independent acquisition (DIA) approaches, provides an opportunity to improve plasma-based biomarker discovery studies because this approach does not rely on precursor intensity for fragmentation selection but rather analyzes all precursors in specified mass ranges. Here, we describe a workflow for SWATH-MS-based analysis of non-depleted plasma including sample preparation, data acquisition, and statistical analysis.

AB - The limitations commonly observed in data-dependent acquisition (DDA) mass spectrometric investigation of non-depleted human plasma are mainly due to the large dynamic concentration range of protein expression. Less abundant proteins are usually masked by highly abundant proteins and are therefore difficult to reliably detect. Sequential window acquisition of all theoretical fragment-ion spectra (SWATH) mass spectrometry (MS), as a representative of data-independent acquisition (DIA) approaches, provides an opportunity to improve plasma-based biomarker discovery studies because this approach does not rely on precursor intensity for fragmentation selection but rather analyzes all precursors in specified mass ranges. Here, we describe a workflow for SWATH-MS-based analysis of non-depleted plasma including sample preparation, data acquisition, and statistical analysis.

KW - Journal Article

U2 - 10.1007/978-1-4939-7057-5_25

DO - 10.1007/978-1-4939-7057-5_25

M3 - SCORING: Journal article

C2 - 28674897

VL - 1619

SP - 373

EP - 383

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -