SWATH Mass Spectrometry for Proteomics of Non-Depleted Plasma
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SWATH Mass Spectrometry for Proteomics of Non-Depleted Plasma. / Krisp, Christoph; Molloy, Mark P.
in: Methods Mol Biol, Jahrgang 1619, 2017, S. 373-383.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - SWATH Mass Spectrometry for Proteomics of Non-Depleted Plasma
AU - Krisp, Christoph
AU - Molloy, Mark P
PY - 2017
Y1 - 2017
N2 - The limitations commonly observed in data-dependent acquisition (DDA) mass spectrometric investigation of non-depleted human plasma are mainly due to the large dynamic concentration range of protein expression. Less abundant proteins are usually masked by highly abundant proteins and are therefore difficult to reliably detect. Sequential window acquisition of all theoretical fragment-ion spectra (SWATH) mass spectrometry (MS), as a representative of data-independent acquisition (DIA) approaches, provides an opportunity to improve plasma-based biomarker discovery studies because this approach does not rely on precursor intensity for fragmentation selection but rather analyzes all precursors in specified mass ranges. Here, we describe a workflow for SWATH-MS-based analysis of non-depleted plasma including sample preparation, data acquisition, and statistical analysis.
AB - The limitations commonly observed in data-dependent acquisition (DDA) mass spectrometric investigation of non-depleted human plasma are mainly due to the large dynamic concentration range of protein expression. Less abundant proteins are usually masked by highly abundant proteins and are therefore difficult to reliably detect. Sequential window acquisition of all theoretical fragment-ion spectra (SWATH) mass spectrometry (MS), as a representative of data-independent acquisition (DIA) approaches, provides an opportunity to improve plasma-based biomarker discovery studies because this approach does not rely on precursor intensity for fragmentation selection but rather analyzes all precursors in specified mass ranges. Here, we describe a workflow for SWATH-MS-based analysis of non-depleted plasma including sample preparation, data acquisition, and statistical analysis.
KW - Journal Article
U2 - 10.1007/978-1-4939-7057-5_25
DO - 10.1007/978-1-4939-7057-5_25
M3 - SCORING: Journal article
C2 - 28674897
VL - 1619
SP - 373
EP - 383
JO - Methods Mol Biol
JF - Methods Mol Biol
SN - 1064-3745
ER -