Support of HUVEC proliferation by pro-angiogenic intermediate CD163+ monocytes/macrophages
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Support of HUVEC proliferation by pro-angiogenic intermediate CD163+ monocytes/macrophages : a co-culture experiment. / Mayer, A; Hiebl, B; Lendlein, A; Jung, F.
In: CLIN HEMORHEOL MICRO, Vol. 49, No. 1-4, 01.01.2011, p. 423-30.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Support of HUVEC proliferation by pro-angiogenic intermediate CD163+ monocytes/macrophages
T2 - a co-culture experiment
AU - Mayer, A
AU - Hiebl, B
AU - Lendlein, A
AU - Jung, F
PY - 2011/1/1
Y1 - 2011/1/1
N2 - So called intermediate (MO2) monocytes/macrophages possess anti-inflammatory properties and express the MO lineage marker CD163. On a hydrophilic, acrylamide-based hydrogel human intermediate (CD14++ CD16+) CD163++ monocytes/macrophages (aMO2) which were angiogenically stimulated, maintained a pro-angiogenic and non-inflammatory status for at least 14 days. Here we explored, whether this aMO2 subset can positively influence the proliferation of human umbilical venous endothelial cells (HUVECs) without switching back into a pro-inflammatory (MO1) phenotype. aMO2 or HUVEC were seeded alone on glass cover slips (0.5 × 10(5) cells / 1.33 cm(2)) in a HUVEC specific cell culture medium (EGM-2) for 3 hrs, 24 hrs and 72 hrs or under co-culture conditions (0.5 × 10(5) HUVEC + 0.25 × 10(5) aMO2 / 1.33 cm(2)) in EGM-2 for the same time window as well (n = 6 each). Under co-culture conditions the numbers of adherent HUVEC per unit area were significantly higher (p < 0.01; 525 ± 52 HUVEC/mm(2)) compared to control mono-cultures (473 ± 76 HUVEC/mm(2)) after 72 hrs of cultivation and showed their typically spread morphology. The aMO2 remained in their subset status and secreted VEGF-A165 without release of pro-inflammatory cytokines until the end of the 72 hrs cultivation time period, thereby supporting the HUVEC proliferation. These in vitro results might indicate that this MO subset can be used as cellular delivery system for pro-angiogenic and non-inflammatory mediators to support the endothelialisation of biomaterials like e.g. cPnBA.
AB - So called intermediate (MO2) monocytes/macrophages possess anti-inflammatory properties and express the MO lineage marker CD163. On a hydrophilic, acrylamide-based hydrogel human intermediate (CD14++ CD16+) CD163++ monocytes/macrophages (aMO2) which were angiogenically stimulated, maintained a pro-angiogenic and non-inflammatory status for at least 14 days. Here we explored, whether this aMO2 subset can positively influence the proliferation of human umbilical venous endothelial cells (HUVECs) without switching back into a pro-inflammatory (MO1) phenotype. aMO2 or HUVEC were seeded alone on glass cover slips (0.5 × 10(5) cells / 1.33 cm(2)) in a HUVEC specific cell culture medium (EGM-2) for 3 hrs, 24 hrs and 72 hrs or under co-culture conditions (0.5 × 10(5) HUVEC + 0.25 × 10(5) aMO2 / 1.33 cm(2)) in EGM-2 for the same time window as well (n = 6 each). Under co-culture conditions the numbers of adherent HUVEC per unit area were significantly higher (p < 0.01; 525 ± 52 HUVEC/mm(2)) compared to control mono-cultures (473 ± 76 HUVEC/mm(2)) after 72 hrs of cultivation and showed their typically spread morphology. The aMO2 remained in their subset status and secreted VEGF-A165 without release of pro-inflammatory cytokines until the end of the 72 hrs cultivation time period, thereby supporting the HUVEC proliferation. These in vitro results might indicate that this MO subset can be used as cellular delivery system for pro-angiogenic and non-inflammatory mediators to support the endothelialisation of biomaterials like e.g. cPnBA.
KW - Antigens, CD
KW - Antigens, Differentiation, Myelomonocytic
KW - Biocompatible Materials
KW - Cell Adhesion
KW - Cell Communication
KW - Cell Division
KW - Cells, Cultured
KW - Coculture Techniques
KW - Glass
KW - Human Umbilical Vein Endothelial Cells
KW - Humans
KW - Macrophages
KW - Monocytes
KW - Neovascularization, Physiologic
KW - Phenotype
KW - Polystyrenes
KW - Receptors, Cell Surface
KW - Tissue Engineering
KW - Vascular Endothelial Growth Factor A
U2 - 10.3233/CH-2011-1492
DO - 10.3233/CH-2011-1492
M3 - SCORING: Journal article
C2 - 22214713
VL - 49
SP - 423
EP - 430
JO - CLIN HEMORHEOL MICRO
JF - CLIN HEMORHEOL MICRO
SN - 1386-0291
IS - 1-4
ER -