Support of HUVEC proliferation by pro-angiogenic intermediate CD163+ monocytes/macrophages

TY - JOUR

T1 - Support of HUVEC proliferation by pro-angiogenic intermediate CD163+ monocytes/macrophages

T2 - a co-culture experiment

AU - Mayer, A

AU - Hiebl, B

AU - Lendlein, A

AU - Jung, F

PY - 2011/1/1

Y1 - 2011/1/1

N2 - So called intermediate (MO2) monocytes/macrophages possess anti-inflammatory properties and express the MO lineage marker CD163. On a hydrophilic, acrylamide-based hydrogel human intermediate (CD14++ CD16+) CD163++ monocytes/macrophages (aMO2) which were angiogenically stimulated, maintained a pro-angiogenic and non-inflammatory status for at least 14 days. Here we explored, whether this aMO2 subset can positively influence the proliferation of human umbilical venous endothelial cells (HUVECs) without switching back into a pro-inflammatory (MO1) phenotype. aMO2 or HUVEC were seeded alone on glass cover slips (0.5 × 10(5) cells / 1.33 cm(2)) in a HUVEC specific cell culture medium (EGM-2) for 3 hrs, 24 hrs and 72 hrs or under co-culture conditions (0.5 × 10(5) HUVEC + 0.25 × 10(5) aMO2 / 1.33 cm(2)) in EGM-2 for the same time window as well (n = 6 each). Under co-culture conditions the numbers of adherent HUVEC per unit area were significantly higher (p < 0.01; 525 ± 52 HUVEC/mm(2)) compared to control mono-cultures (473 ± 76 HUVEC/mm(2)) after 72 hrs of cultivation and showed their typically spread morphology. The aMO2 remained in their subset status and secreted VEGF-A165 without release of pro-inflammatory cytokines until the end of the 72 hrs cultivation time period, thereby supporting the HUVEC proliferation. These in vitro results might indicate that this MO subset can be used as cellular delivery system for pro-angiogenic and non-inflammatory mediators to support the endothelialisation of biomaterials like e.g. cPnBA.

AB - So called intermediate (MO2) monocytes/macrophages possess anti-inflammatory properties and express the MO lineage marker CD163. On a hydrophilic, acrylamide-based hydrogel human intermediate (CD14++ CD16+) CD163++ monocytes/macrophages (aMO2) which were angiogenically stimulated, maintained a pro-angiogenic and non-inflammatory status for at least 14 days. Here we explored, whether this aMO2 subset can positively influence the proliferation of human umbilical venous endothelial cells (HUVECs) without switching back into a pro-inflammatory (MO1) phenotype. aMO2 or HUVEC were seeded alone on glass cover slips (0.5 × 10(5) cells / 1.33 cm(2)) in a HUVEC specific cell culture medium (EGM-2) for 3 hrs, 24 hrs and 72 hrs or under co-culture conditions (0.5 × 10(5) HUVEC + 0.25 × 10(5) aMO2 / 1.33 cm(2)) in EGM-2 for the same time window as well (n = 6 each). Under co-culture conditions the numbers of adherent HUVEC per unit area were significantly higher (p < 0.01; 525 ± 52 HUVEC/mm(2)) compared to control mono-cultures (473 ± 76 HUVEC/mm(2)) after 72 hrs of cultivation and showed their typically spread morphology. The aMO2 remained in their subset status and secreted VEGF-A165 without release of pro-inflammatory cytokines until the end of the 72 hrs cultivation time period, thereby supporting the HUVEC proliferation. These in vitro results might indicate that this MO subset can be used as cellular delivery system for pro-angiogenic and non-inflammatory mediators to support the endothelialisation of biomaterials like e.g. cPnBA.

KW - Antigens, CD

KW - Antigens, Differentiation, Myelomonocytic

KW - Biocompatible Materials

KW - Cell Adhesion

KW - Cell Communication

KW - Cell Division

KW - Cells, Cultured

KW - Coculture Techniques

KW - Glass

KW - Human Umbilical Vein Endothelial Cells

KW - Humans

KW - Macrophages

KW - Monocytes

KW - Neovascularization, Physiologic

KW - Phenotype

KW - Polystyrenes

KW - Receptors, Cell Surface

KW - Tissue Engineering

KW - Vascular Endothelial Growth Factor A

U2 - 10.3233/CH-2011-1492

DO - 10.3233/CH-2011-1492

M3 - SCORING: Journal article

C2 - 22214713

VL - 49

SP - 423

EP - 430

JO - CLIN HEMORHEOL MICRO

JF - CLIN HEMORHEOL MICRO

SN - 1386-0291

IS - 1-4

ER -