Structural and mechanistic aspects influencing the ADAM10-mediated shedding of the prion protein
Standard
Structural and mechanistic aspects influencing the ADAM10-mediated shedding of the prion protein. / Linsenmeier, Luise; Mohammadi, Behnam; Wetzel, Sebastian; Puig, Berta; Jackson, Walker S; Hartmann, Alexander; Uchiyama, Keiji; Sakaguchi, Suehiro; Endres, Kristina; Tatzelt, Jörg; Saftig, Paul; Glatzel, Markus; Altmeppen, Hermann C.
In: MOL NEURODEGENER, Vol. 13, No. 1, 06.04.2018, p. 18.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Structural and mechanistic aspects influencing the ADAM10-mediated shedding of the prion protein
AU - Linsenmeier, Luise
AU - Mohammadi, Behnam
AU - Wetzel, Sebastian
AU - Puig, Berta
AU - Jackson, Walker S
AU - Hartmann, Alexander
AU - Uchiyama, Keiji
AU - Sakaguchi, Suehiro
AU - Endres, Kristina
AU - Tatzelt, Jörg
AU - Saftig, Paul
AU - Glatzel, Markus
AU - Altmeppen, Hermann C
PY - 2018/4/6
Y1 - 2018/4/6
N2 - Background: Proteolytic processing of the prion protein (PrPC) by endogenous proteases generates bioactive membrane-bound and soluble fragments which may help to explain the pleiotropic roles of this protein in the nervous system and in brain diseases. Shedding of almost full-length PrPC into the extracellular space by the metalloprotease ADAM10 is of peculiar relevance since soluble PrP stimulates axonal outgrowth and is protective in neurodegenerative conditions such as Alzheimer’s and prion disease. However, molecular determinates and mechanisms regulating the shedding of PrP are entirely unknown.Methods: We produced an antibody recognizing the neo-epitope of shed PrP generated by ADAM10 in biological samples and used it to study structural and mechanistic aspects affecting the shedding. For this, we investigated genetically modified cellular and murine models by biochemical and morphological approaches.Results: We show that the novel antibody specifically detects shed PrP in cell culture supernatants and murine brain. We demonstrate that ADAM10 is the exclusive sheddase of PrPC in the nervous system and reveal that the glycosylation state and type of membrane-anchorage of PrPC severely affect its shedding. Furthermore, we provide evidence that PrP shedding can be modulated by pharmacological inhibition and stimulation and present data suggesting that shedding is a relevant part of a compensatory network ensuring PrPC homeostasis of the cell.Conclusions: With the new antibody, our study introduces a new tool to reliably investigate PrP-shedding. In addition, this study provides novel and important insight into the regulation of this cleavage event, which is likely to be relevant for diagnostic and therapeutic approaches even beyond neurodegeneration.
AB - Background: Proteolytic processing of the prion protein (PrPC) by endogenous proteases generates bioactive membrane-bound and soluble fragments which may help to explain the pleiotropic roles of this protein in the nervous system and in brain diseases. Shedding of almost full-length PrPC into the extracellular space by the metalloprotease ADAM10 is of peculiar relevance since soluble PrP stimulates axonal outgrowth and is protective in neurodegenerative conditions such as Alzheimer’s and prion disease. However, molecular determinates and mechanisms regulating the shedding of PrP are entirely unknown.Methods: We produced an antibody recognizing the neo-epitope of shed PrP generated by ADAM10 in biological samples and used it to study structural and mechanistic aspects affecting the shedding. For this, we investigated genetically modified cellular and murine models by biochemical and morphological approaches.Results: We show that the novel antibody specifically detects shed PrP in cell culture supernatants and murine brain. We demonstrate that ADAM10 is the exclusive sheddase of PrPC in the nervous system and reveal that the glycosylation state and type of membrane-anchorage of PrPC severely affect its shedding. Furthermore, we provide evidence that PrP shedding can be modulated by pharmacological inhibition and stimulation and present data suggesting that shedding is a relevant part of a compensatory network ensuring PrPC homeostasis of the cell.Conclusions: With the new antibody, our study introduces a new tool to reliably investigate PrP-shedding. In addition, this study provides novel and important insight into the regulation of this cleavage event, which is likely to be relevant for diagnostic and therapeutic approaches even beyond neurodegeneration.
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1186/s13024-018-0248-6
DO - 10.1186/s13024-018-0248-6
M3 - SCORING: Journal article
C2 - 29625583
VL - 13
SP - 18
JO - MOL NEURODEGENER
JF - MOL NEURODEGENER
SN - 1750-1326
IS - 1
ER -