Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response
Standard
Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response. / Huebener, Sina; Tanaka, Charlene K; Uhde, Melanie; Zone, John J; Vensel, William H; Kasarda, Donald D; Beams, Leilani; Briani, Chiara; Green, Peter H R; Altenbach, Susan B; Alaedini, Armin.
In: J PROTEOME RES, Vol. 14, No. 1, 02.01.2015, p. 503-11.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response
AU - Huebener, Sina
AU - Tanaka, Charlene K
AU - Uhde, Melanie
AU - Zone, John J
AU - Vensel, William H
AU - Kasarda, Donald D
AU - Beams, Leilani
AU - Briani, Chiara
AU - Green, Peter H R
AU - Altenbach, Susan B
AU - Alaedini, Armin
PY - 2015/1/2
Y1 - 2015/1/2
N2 - While the antigenic specificity and pathogenic relevance of immunologic reactivity to gluten in celiac disease have been extensively researched, the immune response to nongluten proteins of wheat has not been characterized. We aimed to investigate the level and molecular specificity of antibody response to wheat nongluten proteins in celiac disease. Serum samples from patients and controls were screened for IgG and IgA antibody reactivity to a nongluten protein extract from the wheat cultivar Triticum aestivum Butte 86. Antibodies were further analyzed for reactivity to specific nongluten proteins by two-dimensional gel electrophoresis and immunoblotting. Immunoreactive molecules were identified by tandem mass spectrometry. Compared with healthy controls, patients exhibited significantly higher levels of antibody reactivity to nongluten proteins. The main immunoreactive nongluten antibody target proteins were identified as serpins, purinins, α-amylase/protease inhibitors, globulins, and farinins. Assessment of reactivity toward purified recombinant proteins further confirmed the presence of antibody response to specific antigens. The results demonstrate that, in addition to the well-recognized immune reaction to gluten, celiac disease is associated with a robust humoral response directed at a specific subset of the nongluten proteins of wheat.
AB - While the antigenic specificity and pathogenic relevance of immunologic reactivity to gluten in celiac disease have been extensively researched, the immune response to nongluten proteins of wheat has not been characterized. We aimed to investigate the level and molecular specificity of antibody response to wheat nongluten proteins in celiac disease. Serum samples from patients and controls were screened for IgG and IgA antibody reactivity to a nongluten protein extract from the wheat cultivar Triticum aestivum Butte 86. Antibodies were further analyzed for reactivity to specific nongluten proteins by two-dimensional gel electrophoresis and immunoblotting. Immunoreactive molecules were identified by tandem mass spectrometry. Compared with healthy controls, patients exhibited significantly higher levels of antibody reactivity to nongluten proteins. The main immunoreactive nongluten antibody target proteins were identified as serpins, purinins, α-amylase/protease inhibitors, globulins, and farinins. Assessment of reactivity toward purified recombinant proteins further confirmed the presence of antibody response to specific antigens. The results demonstrate that, in addition to the well-recognized immune reaction to gluten, celiac disease is associated with a robust humoral response directed at a specific subset of the nongluten proteins of wheat.
KW - Antigens
KW - Celiac Disease
KW - Electrophoresis, Gel, Two-Dimensional
KW - Epitopes
KW - Humans
KW - Immunity, Humoral
KW - Immunoblotting
KW - Immunoglobulin A
KW - Immunoglobulin G
KW - Plant Proteins
KW - Tandem Mass Spectrometry
KW - Triticum
KW - Journal Article
KW - Research Support, N.I.H., Extramural
U2 - 10.1021/pr500809b
DO - 10.1021/pr500809b
M3 - SCORING: Journal article
C2 - 25329597
VL - 14
SP - 503
EP - 511
JO - J PROTEOME RES
JF - J PROTEOME RES
SN - 1535-3893
IS - 1
ER -