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Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response

Standard

Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response. / Huebener, Sina; Tanaka, Charlene K; Uhde, Melanie; Zone, John J; Vensel, William H; Kasarda, Donald D; Beams, Leilani; Briani, Chiara; Green, Peter H R; Altenbach, Susan B; Alaedini, Armin.

in: J PROTEOME RES, Jahrgang 14, Nr. 1, 02.01.2015, S. 503-11.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Huebener, S, Tanaka, CK, Uhde, M, Zone, JJ, Vensel, WH, Kasarda, DD, Beams, L, Briani, C, Green, PHR, Altenbach, SB & Alaedini, A 2015, 'Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response', J PROTEOME RES, Jg. 14, Nr. 1, S. 503-11. https://doi.org/10.1021/pr500809b

APA

Huebener, S., Tanaka, C. K., Uhde, M., Zone, J. J., Vensel, W. H., Kasarda, D. D., Beams, L., Briani, C., Green, P. H. R., Altenbach, S. B., & Alaedini, A. (2015). Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response. J PROTEOME RES, 14(1), 503-11. https://doi.org/10.1021/pr500809b

Vancouver

Huebener S, Tanaka CK, Uhde M, Zone JJ, Vensel WH, Kasarda DD et al. Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response. J PROTEOME RES. 2015 Jan 2;14(1):503-11. https://doi.org/10.1021/pr500809b

Bibtex

@article{265f83b63312406aa984b5053500520b,
title = "Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response",
abstract = "While the antigenic specificity and pathogenic relevance of immunologic reactivity to gluten in celiac disease have been extensively researched, the immune response to nongluten proteins of wheat has not been characterized. We aimed to investigate the level and molecular specificity of antibody response to wheat nongluten proteins in celiac disease. Serum samples from patients and controls were screened for IgG and IgA antibody reactivity to a nongluten protein extract from the wheat cultivar Triticum aestivum Butte 86. Antibodies were further analyzed for reactivity to specific nongluten proteins by two-dimensional gel electrophoresis and immunoblotting. Immunoreactive molecules were identified by tandem mass spectrometry. Compared with healthy controls, patients exhibited significantly higher levels of antibody reactivity to nongluten proteins. The main immunoreactive nongluten antibody target proteins were identified as serpins, purinins, α-amylase/protease inhibitors, globulins, and farinins. Assessment of reactivity toward purified recombinant proteins further confirmed the presence of antibody response to specific antigens. The results demonstrate that, in addition to the well-recognized immune reaction to gluten, celiac disease is associated with a robust humoral response directed at a specific subset of the nongluten proteins of wheat. ",
keywords = "Antigens, Celiac Disease, Electrophoresis, Gel, Two-Dimensional, Epitopes, Humans, Immunity, Humoral, Immunoblotting, Immunoglobulin A, Immunoglobulin G, Plant Proteins, Tandem Mass Spectrometry, Triticum, Journal Article, Research Support, N.I.H., Extramural",
author = "Sina Huebener and Tanaka, {Charlene K} and Melanie Uhde and Zone, {John J} and Vensel, {William H} and Kasarda, {Donald D} and Leilani Beams and Chiara Briani and Green, {Peter H R} and Altenbach, {Susan B} and Armin Alaedini",
year = "2015",
month = jan,
day = "2",
doi = "10.1021/pr500809b",
language = "English",
volume = "14",
pages = "503--11",
journal = "J PROTEOME RES",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "1",

}

RIS

TY - JOUR

T1 - Specific nongluten proteins of wheat are novel target antigens in celiac disease humoral response

AU - Huebener, Sina

AU - Tanaka, Charlene K

AU - Uhde, Melanie

AU - Zone, John J

AU - Vensel, William H

AU - Kasarda, Donald D

AU - Beams, Leilani

AU - Briani, Chiara

AU - Green, Peter H R

AU - Altenbach, Susan B

AU - Alaedini, Armin

PY - 2015/1/2

Y1 - 2015/1/2

N2 - While the antigenic specificity and pathogenic relevance of immunologic reactivity to gluten in celiac disease have been extensively researched, the immune response to nongluten proteins of wheat has not been characterized. We aimed to investigate the level and molecular specificity of antibody response to wheat nongluten proteins in celiac disease. Serum samples from patients and controls were screened for IgG and IgA antibody reactivity to a nongluten protein extract from the wheat cultivar Triticum aestivum Butte 86. Antibodies were further analyzed for reactivity to specific nongluten proteins by two-dimensional gel electrophoresis and immunoblotting. Immunoreactive molecules were identified by tandem mass spectrometry. Compared with healthy controls, patients exhibited significantly higher levels of antibody reactivity to nongluten proteins. The main immunoreactive nongluten antibody target proteins were identified as serpins, purinins, α-amylase/protease inhibitors, globulins, and farinins. Assessment of reactivity toward purified recombinant proteins further confirmed the presence of antibody response to specific antigens. The results demonstrate that, in addition to the well-recognized immune reaction to gluten, celiac disease is associated with a robust humoral response directed at a specific subset of the nongluten proteins of wheat.

AB - While the antigenic specificity and pathogenic relevance of immunologic reactivity to gluten in celiac disease have been extensively researched, the immune response to nongluten proteins of wheat has not been characterized. We aimed to investigate the level and molecular specificity of antibody response to wheat nongluten proteins in celiac disease. Serum samples from patients and controls were screened for IgG and IgA antibody reactivity to a nongluten protein extract from the wheat cultivar Triticum aestivum Butte 86. Antibodies were further analyzed for reactivity to specific nongluten proteins by two-dimensional gel electrophoresis and immunoblotting. Immunoreactive molecules were identified by tandem mass spectrometry. Compared with healthy controls, patients exhibited significantly higher levels of antibody reactivity to nongluten proteins. The main immunoreactive nongluten antibody target proteins were identified as serpins, purinins, α-amylase/protease inhibitors, globulins, and farinins. Assessment of reactivity toward purified recombinant proteins further confirmed the presence of antibody response to specific antigens. The results demonstrate that, in addition to the well-recognized immune reaction to gluten, celiac disease is associated with a robust humoral response directed at a specific subset of the nongluten proteins of wheat.

KW - Antigens

KW - Celiac Disease

KW - Electrophoresis, Gel, Two-Dimensional

KW - Epitopes

KW - Humans

KW - Immunity, Humoral

KW - Immunoblotting

KW - Immunoglobulin A

KW - Immunoglobulin G

KW - Plant Proteins

KW - Tandem Mass Spectrometry

KW - Triticum

KW - Journal Article

KW - Research Support, N.I.H., Extramural

U2 - 10.1021/pr500809b

DO - 10.1021/pr500809b

M3 - SCORING: Journal article

C2 - 25329597

VL - 14

SP - 503

EP - 511

JO - J PROTEOME RES

JF - J PROTEOME RES

SN - 1535-3893

IS - 1

ER -