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Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice

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Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice. / Beller, Alexander; Kruglov, Andrey; Durek, Pawel; von Goetze, Victoria; Werner, Katharina; Heinz, Gitta Anne; Ninnemann, Justus; Lehmann, Katrin; Maier, René; Hoffmann, Ute; Riedel, René; Heiking, Kevin; Zimmermann, Jakob; Siegmund, Britta; Mashreghi, Mir-Farzin; Radbruch, Andreas; Chang, Hyun-Dong.

In: EUR J IMMUNOL, Vol. 50, No. 6, 06.2020, p. 783-794.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Beller, A, Kruglov, A, Durek, P, von Goetze, V, Werner, K, Heinz, GA, Ninnemann, J, Lehmann, K, Maier, R, Hoffmann, U, Riedel, R, Heiking, K, Zimmermann, J, Siegmund, B, Mashreghi, M-F, Radbruch, A & Chang, H-D 2020, 'Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice', EUR J IMMUNOL, vol. 50, no. 6, pp. 783-794. https://doi.org/10.1002/eji.201948474

APA

Beller, A., Kruglov, A., Durek, P., von Goetze, V., Werner, K., Heinz, G. A., Ninnemann, J., Lehmann, K., Maier, R., Hoffmann, U., Riedel, R., Heiking, K., Zimmermann, J., Siegmund, B., Mashreghi, M-F., Radbruch, A., & Chang, H-D. (2020). Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice. EUR J IMMUNOL, 50(6), 783-794. https://doi.org/10.1002/eji.201948474

Vancouver

Beller A, Kruglov A, Durek P, von Goetze V, Werner K, Heinz GA et al. Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice. EUR J IMMUNOL. 2020 Jun;50(6):783-794. https://doi.org/10.1002/eji.201948474

Bibtex

@article{c1469fe72d9a440bb06f1a11150ab611,
title = "Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice",
abstract = "In humans and mice, mucosal immune responses are dominated by IgA antibodies and the cytokine TGF-β, suppressing unwanted immune reactions but also targeting Ig class switching to IgA. It had been suggested that eosinophils promote the generation and maintenance of mucosal IgA-expressing plasma cells. Here, we demonstrate that not eosinophils, but specific bacteria determine mucosal IgA production. Co-housing of eosinophil-deficient mice with mice having high intestinal IgA levels, as well as the intentional microbiota transfer induces TGF-β expression in intestinal T follicular helper cells, thereby promoting IgA class switching in Peyer's patches, enhancing IgA+ plasma cell numbers in the small intestinal lamina propria and levels of mucosal IgA. We show that bacteria highly enriched for the genus Anaeroplasma are sufficient to induce these changes and enhance IgA levels when adoptively transferred. Thus, specific members of the intestinal microbiota and not the microbiota as such regulate gut homeostasis, by promoting the expression of immune-regulatory TGF-β and of mucosal IgA.",
keywords = "Animals, Gastrointestinal Microbiome/immunology, Immunity, Mucosal, Immunoglobulin A/immunology, Intestinal Mucosa/immunology, Mice, Mice, Inbred BALB C, Mice, Knockout, Peyer's Patches/immunology, T-Lymphocytes, Helper-Inducer/immunology, Tenericutes/immunology",
author = "Alexander Beller and Andrey Kruglov and Pawel Durek and {von Goetze}, Victoria and Katharina Werner and Heinz, {Gitta Anne} and Justus Ninnemann and Katrin Lehmann and Ren{\'e} Maier and Ute Hoffmann and Ren{\'e} Riedel and Kevin Heiking and Jakob Zimmermann and Britta Siegmund and Mir-Farzin Mashreghi and Andreas Radbruch and Hyun-Dong Chang",
note = "{\textcopyright} 2020 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2020",
month = jun,
doi = "10.1002/eji.201948474",
language = "English",
volume = "50",
pages = "783--794",
journal = "EUR J IMMUNOL",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag GmbH",
number = "6",

}

RIS

TY - JOUR

T1 - Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice

AU - Beller, Alexander

AU - Kruglov, Andrey

AU - Durek, Pawel

AU - von Goetze, Victoria

AU - Werner, Katharina

AU - Heinz, Gitta Anne

AU - Ninnemann, Justus

AU - Lehmann, Katrin

AU - Maier, René

AU - Hoffmann, Ute

AU - Riedel, René

AU - Heiking, Kevin

AU - Zimmermann, Jakob

AU - Siegmund, Britta

AU - Mashreghi, Mir-Farzin

AU - Radbruch, Andreas

AU - Chang, Hyun-Dong

N1 - © 2020 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2020/6

Y1 - 2020/6

N2 - In humans and mice, mucosal immune responses are dominated by IgA antibodies and the cytokine TGF-β, suppressing unwanted immune reactions but also targeting Ig class switching to IgA. It had been suggested that eosinophils promote the generation and maintenance of mucosal IgA-expressing plasma cells. Here, we demonstrate that not eosinophils, but specific bacteria determine mucosal IgA production. Co-housing of eosinophil-deficient mice with mice having high intestinal IgA levels, as well as the intentional microbiota transfer induces TGF-β expression in intestinal T follicular helper cells, thereby promoting IgA class switching in Peyer's patches, enhancing IgA+ plasma cell numbers in the small intestinal lamina propria and levels of mucosal IgA. We show that bacteria highly enriched for the genus Anaeroplasma are sufficient to induce these changes and enhance IgA levels when adoptively transferred. Thus, specific members of the intestinal microbiota and not the microbiota as such regulate gut homeostasis, by promoting the expression of immune-regulatory TGF-β and of mucosal IgA.

AB - In humans and mice, mucosal immune responses are dominated by IgA antibodies and the cytokine TGF-β, suppressing unwanted immune reactions but also targeting Ig class switching to IgA. It had been suggested that eosinophils promote the generation and maintenance of mucosal IgA-expressing plasma cells. Here, we demonstrate that not eosinophils, but specific bacteria determine mucosal IgA production. Co-housing of eosinophil-deficient mice with mice having high intestinal IgA levels, as well as the intentional microbiota transfer induces TGF-β expression in intestinal T follicular helper cells, thereby promoting IgA class switching in Peyer's patches, enhancing IgA+ plasma cell numbers in the small intestinal lamina propria and levels of mucosal IgA. We show that bacteria highly enriched for the genus Anaeroplasma are sufficient to induce these changes and enhance IgA levels when adoptively transferred. Thus, specific members of the intestinal microbiota and not the microbiota as such regulate gut homeostasis, by promoting the expression of immune-regulatory TGF-β and of mucosal IgA.

KW - Animals

KW - Gastrointestinal Microbiome/immunology

KW - Immunity, Mucosal

KW - Immunoglobulin A/immunology

KW - Intestinal Mucosa/immunology

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Knockout

KW - Peyer's Patches/immunology

KW - T-Lymphocytes, Helper-Inducer/immunology

KW - Tenericutes/immunology

U2 - 10.1002/eji.201948474

DO - 10.1002/eji.201948474

M3 - SCORING: Journal article

C2 - 32065660

VL - 50

SP - 783

EP - 794

JO - EUR J IMMUNOL

JF - EUR J IMMUNOL

SN - 0014-2980

IS - 6

ER -