Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice
Standard
Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice. / Beller, Alexander; Kruglov, Andrey; Durek, Pawel; von Goetze, Victoria; Werner, Katharina; Heinz, Gitta Anne; Ninnemann, Justus; Lehmann, Katrin; Maier, René; Hoffmann, Ute; Riedel, René; Heiking, Kevin; Zimmermann, Jakob; Siegmund, Britta; Mashreghi, Mir-Farzin; Radbruch, Andreas; Chang, Hyun-Dong.
in: EUR J IMMUNOL, Jahrgang 50, Nr. 6, 06.2020, S. 783-794.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice
AU - Beller, Alexander
AU - Kruglov, Andrey
AU - Durek, Pawel
AU - von Goetze, Victoria
AU - Werner, Katharina
AU - Heinz, Gitta Anne
AU - Ninnemann, Justus
AU - Lehmann, Katrin
AU - Maier, René
AU - Hoffmann, Ute
AU - Riedel, René
AU - Heiking, Kevin
AU - Zimmermann, Jakob
AU - Siegmund, Britta
AU - Mashreghi, Mir-Farzin
AU - Radbruch, Andreas
AU - Chang, Hyun-Dong
N1 - © 2020 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2020/6
Y1 - 2020/6
N2 - In humans and mice, mucosal immune responses are dominated by IgA antibodies and the cytokine TGF-β, suppressing unwanted immune reactions but also targeting Ig class switching to IgA. It had been suggested that eosinophils promote the generation and maintenance of mucosal IgA-expressing plasma cells. Here, we demonstrate that not eosinophils, but specific bacteria determine mucosal IgA production. Co-housing of eosinophil-deficient mice with mice having high intestinal IgA levels, as well as the intentional microbiota transfer induces TGF-β expression in intestinal T follicular helper cells, thereby promoting IgA class switching in Peyer's patches, enhancing IgA+ plasma cell numbers in the small intestinal lamina propria and levels of mucosal IgA. We show that bacteria highly enriched for the genus Anaeroplasma are sufficient to induce these changes and enhance IgA levels when adoptively transferred. Thus, specific members of the intestinal microbiota and not the microbiota as such regulate gut homeostasis, by promoting the expression of immune-regulatory TGF-β and of mucosal IgA.
AB - In humans and mice, mucosal immune responses are dominated by IgA antibodies and the cytokine TGF-β, suppressing unwanted immune reactions but also targeting Ig class switching to IgA. It had been suggested that eosinophils promote the generation and maintenance of mucosal IgA-expressing plasma cells. Here, we demonstrate that not eosinophils, but specific bacteria determine mucosal IgA production. Co-housing of eosinophil-deficient mice with mice having high intestinal IgA levels, as well as the intentional microbiota transfer induces TGF-β expression in intestinal T follicular helper cells, thereby promoting IgA class switching in Peyer's patches, enhancing IgA+ plasma cell numbers in the small intestinal lamina propria and levels of mucosal IgA. We show that bacteria highly enriched for the genus Anaeroplasma are sufficient to induce these changes and enhance IgA levels when adoptively transferred. Thus, specific members of the intestinal microbiota and not the microbiota as such regulate gut homeostasis, by promoting the expression of immune-regulatory TGF-β and of mucosal IgA.
KW - Animals
KW - Gastrointestinal Microbiome/immunology
KW - Immunity, Mucosal
KW - Immunoglobulin A/immunology
KW - Intestinal Mucosa/immunology
KW - Mice
KW - Mice, Inbred BALB C
KW - Mice, Knockout
KW - Peyer's Patches/immunology
KW - T-Lymphocytes, Helper-Inducer/immunology
KW - Tenericutes/immunology
U2 - 10.1002/eji.201948474
DO - 10.1002/eji.201948474
M3 - SCORING: Journal article
C2 - 32065660
VL - 50
SP - 783
EP - 794
JO - EUR J IMMUNOL
JF - EUR J IMMUNOL
SN - 0014-2980
IS - 6
ER -