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Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation

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Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation. / Mirza, Nora; Prokop, Lea; Kowalewski, Daniel; Gouttefangeas, Cécile; Faul, Christoph; Bethge, Wolfgang A; Vogel, Wichard; Kanz, Lothar; Rammensee, Hans-Georg; Haen, Sebastian P.

In: TRANSPL IMMUNOL, Vol. 36, 05.2016, p. 25-31.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Mirza, N, Prokop, L, Kowalewski, D, Gouttefangeas, C, Faul, C, Bethge, WA, Vogel, W, Kanz, L, Rammensee, H-G & Haen, SP 2016, 'Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation', TRANSPL IMMUNOL, vol. 36, pp. 25-31. https://doi.org/10.1016/j.trim.2016.03.003

APA

Mirza, N., Prokop, L., Kowalewski, D., Gouttefangeas, C., Faul, C., Bethge, W. A., Vogel, W., Kanz, L., Rammensee, H-G., & Haen, S. P. (2016). Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation. TRANSPL IMMUNOL, 36, 25-31. https://doi.org/10.1016/j.trim.2016.03.003

Vancouver

Mirza N, Prokop L, Kowalewski D, Gouttefangeas C, Faul C, Bethge WA et al. Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation. TRANSPL IMMUNOL. 2016 May;36:25-31. https://doi.org/10.1016/j.trim.2016.03.003

Bibtex

@article{2e23f99b90c9458b97d2135944ccb249,
title = "Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation",
abstract = "BACKGROUND: Heat shock proteins (HSP) are highly conserved immunogenic proteins serving as potent danger signals. They are upregulated under stress conditions like fever and hypoxia. Extracellular HSP are involved in antigen presentation, cytokine release and maturation of antigen presenting cells.METHODS: The release of the inducible members of the HSP70 family, Hsp72 and Hsp70B', into the serum of 20 patients undergoing allogeneic hematopoietic cell transplantation and 20 healthy donors was evaluated using enzyme linked immunosorbent assay (ELISA) kits.RESULTS: Eight patients (40%) did not receive anti-thymocyte globulin (ATG) for prophylaxis of graft versus host disease (GvHD). These patients had no detectable or low serum levels of Hsp72 (n=3, 0.03 to 1.92ng/ml) which were in line with levels detected in 20 healthy individuals (p=0.07). Measurable HSP was not associated with any medication or transplantation-related procedures. In twelve patients (60%) receiving ATG, detected high levels of HSP reflected cross-reactivity of the rabbit-derived ATG with the anti-rabbit antibody used in the ELISA.CONCLUSIONS: Assumed HSP70 expression detected such ELISA has to be regarded carefully after ATG application. Neither radiochemotherapy, nor inflammation or sepsis during aplasia induced HSP70 release into the serum. Thus, soluble HSP70 may not be involved in the pathogenesis of acute GvHD.",
keywords = "Adult, Aged, Animals, Biomarkers/blood, Cross Reactions, Female, Graft vs Host Disease/diagnosis, HSP70 Heat-Shock Proteins/blood, HSP72 Heat-Shock Proteins/blood, Hematopoietic Stem Cell Transplantation, Humans, Male, Middle Aged, Rabbits, Transplantation, Homologous",
author = "Nora Mirza and Lea Prokop and Daniel Kowalewski and C{\'e}cile Gouttefangeas and Christoph Faul and Bethge, {Wolfgang A} and Wichard Vogel and Lothar Kanz and Hans-Georg Rammensee and Haen, {Sebastian P}",
note = "Copyright {\textcopyright} 2016 Elsevier B.V. All rights reserved.",
year = "2016",
month = may,
doi = "10.1016/j.trim.2016.03.003",
language = "English",
volume = "36",
pages = "25--31",
journal = "TRANSPL IMMUNOL",
issn = "0966-3274",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation

AU - Mirza, Nora

AU - Prokop, Lea

AU - Kowalewski, Daniel

AU - Gouttefangeas, Cécile

AU - Faul, Christoph

AU - Bethge, Wolfgang A

AU - Vogel, Wichard

AU - Kanz, Lothar

AU - Rammensee, Hans-Georg

AU - Haen, Sebastian P

N1 - Copyright © 2016 Elsevier B.V. All rights reserved.

PY - 2016/5

Y1 - 2016/5

N2 - BACKGROUND: Heat shock proteins (HSP) are highly conserved immunogenic proteins serving as potent danger signals. They are upregulated under stress conditions like fever and hypoxia. Extracellular HSP are involved in antigen presentation, cytokine release and maturation of antigen presenting cells.METHODS: The release of the inducible members of the HSP70 family, Hsp72 and Hsp70B', into the serum of 20 patients undergoing allogeneic hematopoietic cell transplantation and 20 healthy donors was evaluated using enzyme linked immunosorbent assay (ELISA) kits.RESULTS: Eight patients (40%) did not receive anti-thymocyte globulin (ATG) for prophylaxis of graft versus host disease (GvHD). These patients had no detectable or low serum levels of Hsp72 (n=3, 0.03 to 1.92ng/ml) which were in line with levels detected in 20 healthy individuals (p=0.07). Measurable HSP was not associated with any medication or transplantation-related procedures. In twelve patients (60%) receiving ATG, detected high levels of HSP reflected cross-reactivity of the rabbit-derived ATG with the anti-rabbit antibody used in the ELISA.CONCLUSIONS: Assumed HSP70 expression detected such ELISA has to be regarded carefully after ATG application. Neither radiochemotherapy, nor inflammation or sepsis during aplasia induced HSP70 release into the serum. Thus, soluble HSP70 may not be involved in the pathogenesis of acute GvHD.

AB - BACKGROUND: Heat shock proteins (HSP) are highly conserved immunogenic proteins serving as potent danger signals. They are upregulated under stress conditions like fever and hypoxia. Extracellular HSP are involved in antigen presentation, cytokine release and maturation of antigen presenting cells.METHODS: The release of the inducible members of the HSP70 family, Hsp72 and Hsp70B', into the serum of 20 patients undergoing allogeneic hematopoietic cell transplantation and 20 healthy donors was evaluated using enzyme linked immunosorbent assay (ELISA) kits.RESULTS: Eight patients (40%) did not receive anti-thymocyte globulin (ATG) for prophylaxis of graft versus host disease (GvHD). These patients had no detectable or low serum levels of Hsp72 (n=3, 0.03 to 1.92ng/ml) which were in line with levels detected in 20 healthy individuals (p=0.07). Measurable HSP was not associated with any medication or transplantation-related procedures. In twelve patients (60%) receiving ATG, detected high levels of HSP reflected cross-reactivity of the rabbit-derived ATG with the anti-rabbit antibody used in the ELISA.CONCLUSIONS: Assumed HSP70 expression detected such ELISA has to be regarded carefully after ATG application. Neither radiochemotherapy, nor inflammation or sepsis during aplasia induced HSP70 release into the serum. Thus, soluble HSP70 may not be involved in the pathogenesis of acute GvHD.

KW - Adult

KW - Aged

KW - Animals

KW - Biomarkers/blood

KW - Cross Reactions

KW - Female

KW - Graft vs Host Disease/diagnosis

KW - HSP70 Heat-Shock Proteins/blood

KW - HSP72 Heat-Shock Proteins/blood

KW - Hematopoietic Stem Cell Transplantation

KW - Humans

KW - Male

KW - Middle Aged

KW - Rabbits

KW - Transplantation, Homologous

U2 - 10.1016/j.trim.2016.03.003

DO - 10.1016/j.trim.2016.03.003

M3 - SCORING: Journal article

C2 - 27020764

VL - 36

SP - 25

EP - 31

JO - TRANSPL IMMUNOL

JF - TRANSPL IMMUNOL

SN - 0966-3274

ER -