Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation
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Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation. / Mirza, Nora; Prokop, Lea; Kowalewski, Daniel; Gouttefangeas, Cécile; Faul, Christoph; Bethge, Wolfgang A; Vogel, Wichard; Kanz, Lothar; Rammensee, Hans-Georg; Haen, Sebastian P.
in: TRANSPL IMMUNOL, Jahrgang 36, 05.2016, S. 25-31.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation
AU - Mirza, Nora
AU - Prokop, Lea
AU - Kowalewski, Daniel
AU - Gouttefangeas, Cécile
AU - Faul, Christoph
AU - Bethge, Wolfgang A
AU - Vogel, Wichard
AU - Kanz, Lothar
AU - Rammensee, Hans-Georg
AU - Haen, Sebastian P
N1 - Copyright © 2016 Elsevier B.V. All rights reserved.
PY - 2016/5
Y1 - 2016/5
N2 - BACKGROUND: Heat shock proteins (HSP) are highly conserved immunogenic proteins serving as potent danger signals. They are upregulated under stress conditions like fever and hypoxia. Extracellular HSP are involved in antigen presentation, cytokine release and maturation of antigen presenting cells.METHODS: The release of the inducible members of the HSP70 family, Hsp72 and Hsp70B', into the serum of 20 patients undergoing allogeneic hematopoietic cell transplantation and 20 healthy donors was evaluated using enzyme linked immunosorbent assay (ELISA) kits.RESULTS: Eight patients (40%) did not receive anti-thymocyte globulin (ATG) for prophylaxis of graft versus host disease (GvHD). These patients had no detectable or low serum levels of Hsp72 (n=3, 0.03 to 1.92ng/ml) which were in line with levels detected in 20 healthy individuals (p=0.07). Measurable HSP was not associated with any medication or transplantation-related procedures. In twelve patients (60%) receiving ATG, detected high levels of HSP reflected cross-reactivity of the rabbit-derived ATG with the anti-rabbit antibody used in the ELISA.CONCLUSIONS: Assumed HSP70 expression detected such ELISA has to be regarded carefully after ATG application. Neither radiochemotherapy, nor inflammation or sepsis during aplasia induced HSP70 release into the serum. Thus, soluble HSP70 may not be involved in the pathogenesis of acute GvHD.
AB - BACKGROUND: Heat shock proteins (HSP) are highly conserved immunogenic proteins serving as potent danger signals. They are upregulated under stress conditions like fever and hypoxia. Extracellular HSP are involved in antigen presentation, cytokine release and maturation of antigen presenting cells.METHODS: The release of the inducible members of the HSP70 family, Hsp72 and Hsp70B', into the serum of 20 patients undergoing allogeneic hematopoietic cell transplantation and 20 healthy donors was evaluated using enzyme linked immunosorbent assay (ELISA) kits.RESULTS: Eight patients (40%) did not receive anti-thymocyte globulin (ATG) for prophylaxis of graft versus host disease (GvHD). These patients had no detectable or low serum levels of Hsp72 (n=3, 0.03 to 1.92ng/ml) which were in line with levels detected in 20 healthy individuals (p=0.07). Measurable HSP was not associated with any medication or transplantation-related procedures. In twelve patients (60%) receiving ATG, detected high levels of HSP reflected cross-reactivity of the rabbit-derived ATG with the anti-rabbit antibody used in the ELISA.CONCLUSIONS: Assumed HSP70 expression detected such ELISA has to be regarded carefully after ATG application. Neither radiochemotherapy, nor inflammation or sepsis during aplasia induced HSP70 release into the serum. Thus, soluble HSP70 may not be involved in the pathogenesis of acute GvHD.
KW - Adult
KW - Aged
KW - Animals
KW - Biomarkers/blood
KW - Cross Reactions
KW - Female
KW - Graft vs Host Disease/diagnosis
KW - HSP70 Heat-Shock Proteins/blood
KW - HSP72 Heat-Shock Proteins/blood
KW - Hematopoietic Stem Cell Transplantation
KW - Humans
KW - Male
KW - Middle Aged
KW - Rabbits
KW - Transplantation, Homologous
U2 - 10.1016/j.trim.2016.03.003
DO - 10.1016/j.trim.2016.03.003
M3 - SCORING: Journal article
C2 - 27020764
VL - 36
SP - 25
EP - 31
JO - TRANSPL IMMUNOL
JF - TRANSPL IMMUNOL
SN - 0966-3274
ER -