Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers.
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Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers. / Schneider, Robert; Ader, Christian; Lange, Adam; Giller, Karin; Hornig, Sönke; Pongs, Olaf; Becker, Stefan; Baldus, Marc.
In: J AM CHEM SOC, Vol. 130, No. 23, 23, 2008, p. 7427-7435.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers.
AU - Schneider, Robert
AU - Ader, Christian
AU - Lange, Adam
AU - Giller, Karin
AU - Hornig, Sönke
AU - Pongs, Olaf
AU - Becker, Stefan
AU - Baldus, Marc
PY - 2008
Y1 - 2008
N2 - We show that solid-state NMR can be used to investigate the structure and dynamics of a chimeric potassium channel, KcsA-Kv1.3, in lipid bilayers. Sequential resonance assignments were obtained using a combination of (15)N- (13)C and (13)C- (13)C correlation experiments conducted on fully labeled and reverse-labeled as well as C-terminally truncated samples. Comparison of our results with those from X-ray crystallography and solution-state NMR in micelles on the closely related KcsA K (+) channel provides insight into the mechanism of ion channel selectivity and underlines the important role of the lipid environment for membrane protein structure and function.
AB - We show that solid-state NMR can be used to investigate the structure and dynamics of a chimeric potassium channel, KcsA-Kv1.3, in lipid bilayers. Sequential resonance assignments were obtained using a combination of (15)N- (13)C and (13)C- (13)C correlation experiments conducted on fully labeled and reverse-labeled as well as C-terminally truncated samples. Comparison of our results with those from X-ray crystallography and solution-state NMR in micelles on the closely related KcsA K (+) channel provides insight into the mechanism of ion channel selectivity and underlines the important role of the lipid environment for membrane protein structure and function.
M3 - SCORING: Zeitschriftenaufsatz
VL - 130
SP - 7427
EP - 7435
JO - J AM CHEM SOC
JF - J AM CHEM SOC
SN - 0002-7863
IS - 23
M1 - 23
ER -