Simultaneous determination of oxalate, citrate and sulfate in children's plasma with ion chromatography.
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Simultaneous determination of oxalate, citrate and sulfate in children's plasma with ion chromatography. / Hoppe, B; Kemper, Markus J.; Hvizd, M G; Sailer, D E; Langman, C B.
In: KIDNEY INT, Vol. 53, No. 5, 5, 1998, p. 1348-1352.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Simultaneous determination of oxalate, citrate and sulfate in children's plasma with ion chromatography.
AU - Hoppe, B
AU - Kemper, Markus J.
AU - Hvizd, M G
AU - Sailer, D E
AU - Langman, C B
PY - 1998
Y1 - 1998
N2 - To improve our understanding of both diagnosis and treatment of diseases of oxalate metabolism, we first set out to establish a new ion-chromatographic method to determine normal plasma levels of oxalate, citrate and sulfate from single plasma samples. In 50 infants and children (23 girls, 27 boys, aged 0.2 to 17 years) with normal renal function, blood was drawn in Li-heparin tubes, placed on ice and preserved immediately with 40 microliters M HCl/ml plasma in two ultracentrifugation steps. For measurement, plasma was injected onto an ion chromatography system with NaOH as the mobile phase, and then run as a linear gradient from 5 mM to 52.5 mM over 21 minutes. Analysis yielded measurable and reproducible oxalate (6.43 +/- 1.06 microM/liter), citrate (79.3 +/- 27.4 microM/liter) and sulfate (235.0 +/- 85.3 microM/liter) levels, without any age and gender specific differences. The least detectable plasma oxalate level was < 0.3 microM with a high reliability and reproducibility (coefficient of variance 1.95 to 4.75%). In conclusion, we established a reproducible, precise method to determine the relevant plasma anions involved in mineral metabolism, which heretofore have not been easily measurable. Studies of diseases of oxalate and citrate metabolism are ongoing on the basis of the normal plasma values achieved in this study.
AB - To improve our understanding of both diagnosis and treatment of diseases of oxalate metabolism, we first set out to establish a new ion-chromatographic method to determine normal plasma levels of oxalate, citrate and sulfate from single plasma samples. In 50 infants and children (23 girls, 27 boys, aged 0.2 to 17 years) with normal renal function, blood was drawn in Li-heparin tubes, placed on ice and preserved immediately with 40 microliters M HCl/ml plasma in two ultracentrifugation steps. For measurement, plasma was injected onto an ion chromatography system with NaOH as the mobile phase, and then run as a linear gradient from 5 mM to 52.5 mM over 21 minutes. Analysis yielded measurable and reproducible oxalate (6.43 +/- 1.06 microM/liter), citrate (79.3 +/- 27.4 microM/liter) and sulfate (235.0 +/- 85.3 microM/liter) levels, without any age and gender specific differences. The least detectable plasma oxalate level was < 0.3 microM with a high reliability and reproducibility (coefficient of variance 1.95 to 4.75%). In conclusion, we established a reproducible, precise method to determine the relevant plasma anions involved in mineral metabolism, which heretofore have not been easily measurable. Studies of diseases of oxalate and citrate metabolism are ongoing on the basis of the normal plasma values achieved in this study.
KW - Humans
KW - Male
KW - Female
KW - Adolescent
KW - Child
KW - Reproducibility of Results
KW - Child, Preschool
KW - Infant
KW - Reference Values
KW - Evaluation Studies as Topic
KW - Blood Chemical Analysis/methods/statistics & numerical data
KW - Chromatography, Ion Exchange/methods/statistics & numerical data
KW - Citric Acid/blood
KW - Oxalates/blood
KW - Oxalic Acid
KW - Sulfates/blood
KW - Humans
KW - Male
KW - Female
KW - Adolescent
KW - Child
KW - Reproducibility of Results
KW - Child, Preschool
KW - Infant
KW - Reference Values
KW - Evaluation Studies as Topic
KW - Blood Chemical Analysis/methods/statistics & numerical data
KW - Chromatography, Ion Exchange/methods/statistics & numerical data
KW - Citric Acid/blood
KW - Oxalates/blood
KW - Oxalic Acid
KW - Sulfates/blood
M3 - SCORING: Journal article
VL - 53
SP - 1348
EP - 1352
JO - KIDNEY INT
JF - KIDNEY INT
SN - 0085-2538
IS - 5
M1 - 5
ER -