Simultaneous determination of oxalate, citrate and sulfate in children's plasma with ion chromatography.

TY - JOUR

T1 - Simultaneous determination of oxalate, citrate and sulfate in children's plasma with ion chromatography.

AU - Hoppe, B

AU - Kemper, Markus J.

AU - Hvizd, M G

AU - Sailer, D E

AU - Langman, C B

PY - 1998

Y1 - 1998

N2 - To improve our understanding of both diagnosis and treatment of diseases of oxalate metabolism, we first set out to establish a new ion-chromatographic method to determine normal plasma levels of oxalate, citrate and sulfate from single plasma samples. In 50 infants and children (23 girls, 27 boys, aged 0.2 to 17 years) with normal renal function, blood was drawn in Li-heparin tubes, placed on ice and preserved immediately with 40 microliters M HCl/ml plasma in two ultracentrifugation steps. For measurement, plasma was injected onto an ion chromatography system with NaOH as the mobile phase, and then run as a linear gradient from 5 mM to 52.5 mM over 21 minutes. Analysis yielded measurable and reproducible oxalate (6.43 +/- 1.06 microM/liter), citrate (79.3 +/- 27.4 microM/liter) and sulfate (235.0 +/- 85.3 microM/liter) levels, without any age and gender specific differences. The least detectable plasma oxalate level was < 0.3 microM with a high reliability and reproducibility (coefficient of variance 1.95 to 4.75%). In conclusion, we established a reproducible, precise method to determine the relevant plasma anions involved in mineral metabolism, which heretofore have not been easily measurable. Studies of diseases of oxalate and citrate metabolism are ongoing on the basis of the normal plasma values achieved in this study.

AB - To improve our understanding of both diagnosis and treatment of diseases of oxalate metabolism, we first set out to establish a new ion-chromatographic method to determine normal plasma levels of oxalate, citrate and sulfate from single plasma samples. In 50 infants and children (23 girls, 27 boys, aged 0.2 to 17 years) with normal renal function, blood was drawn in Li-heparin tubes, placed on ice and preserved immediately with 40 microliters M HCl/ml plasma in two ultracentrifugation steps. For measurement, plasma was injected onto an ion chromatography system with NaOH as the mobile phase, and then run as a linear gradient from 5 mM to 52.5 mM over 21 minutes. Analysis yielded measurable and reproducible oxalate (6.43 +/- 1.06 microM/liter), citrate (79.3 +/- 27.4 microM/liter) and sulfate (235.0 +/- 85.3 microM/liter) levels, without any age and gender specific differences. The least detectable plasma oxalate level was < 0.3 microM with a high reliability and reproducibility (coefficient of variance 1.95 to 4.75%). In conclusion, we established a reproducible, precise method to determine the relevant plasma anions involved in mineral metabolism, which heretofore have not been easily measurable. Studies of diseases of oxalate and citrate metabolism are ongoing on the basis of the normal plasma values achieved in this study.

KW - Humans

KW - Male

KW - Female

KW - Adolescent

KW - Child

KW - Reproducibility of Results

KW - Child, Preschool

KW - Infant

KW - Reference Values

KW - Evaluation Studies as Topic

KW - Blood Chemical Analysis/methods/statistics & numerical data

KW - Chromatography, Ion Exchange/methods/statistics & numerical data

KW - Citric Acid/blood

KW - Oxalates/blood

KW - Oxalic Acid

KW - Sulfates/blood

KW - Humans

KW - Male

KW - Female

KW - Adolescent

KW - Child

KW - Reproducibility of Results

KW - Child, Preschool

KW - Infant

KW - Reference Values

KW - Evaluation Studies as Topic

KW - Blood Chemical Analysis/methods/statistics & numerical data

KW - Chromatography, Ion Exchange/methods/statistics & numerical data

KW - Citric Acid/blood

KW - Oxalates/blood

KW - Oxalic Acid

KW - Sulfates/blood

M3 - SCORING: Journal article

VL - 53

SP - 1348

EP - 1352

JO - KIDNEY INT

JF - KIDNEY INT

SN - 0085-2538

IS - 5

M1 - 5

ER -