Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria
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Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria. / Neo, Melissa; Morris, David G; Kuklik, Pawel; Lau, Dennis H; Dimitri, Hany; Lim, Wei-Wen; Sanders, Prashanthan; Saint, David A.
In: CAN J PHYSIOL PHARM, Vol. 94, No. 5, 05.2016, p. 563-569.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria
AU - Neo, Melissa
AU - Morris, David G
AU - Kuklik, Pawel
AU - Lau, Dennis H
AU - Dimitri, Hany
AU - Lim, Wei-Wen
AU - Sanders, Prashanthan
AU - Saint, David A
PY - 2016/5
Y1 - 2016/5
N2 - We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 × 10 silver chloride electrodes, 0.1 mm diameter and 0.1 mm pitch). A glass microelectrode (100 MΩ) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease.
AB - We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 × 10 silver chloride electrodes, 0.1 mm diameter and 0.1 mm pitch). A glass microelectrode (100 MΩ) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease.
KW - Action Potentials
KW - Animals
KW - Arrhythmias, Cardiac/physiopathology
KW - Atrial Function
KW - Electrophysiologic Techniques, Cardiac/instrumentation
KW - Functional Neuroimaging
KW - Heart Atria/innervation
KW - In Vitro Techniques
KW - Intracellular Membranes/physiology
KW - Male
KW - Membrane Potentials
KW - Microarray Analysis
KW - Microelectrodes
KW - Neural Conduction
KW - Pilot Projects
KW - Rats, Sprague-Dawley
KW - Reproducibility of Results
KW - Tachycardia/physiopathology
U2 - 10.1139/cjpp-2015-0194
DO - 10.1139/cjpp-2015-0194
M3 - SCORING: Journal article
C2 - 26771118
VL - 94
SP - 563
EP - 569
JO - CAN J PHYSIOL PHARM
JF - CAN J PHYSIOL PHARM
SN - 0008-4212
IS - 5
ER -