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Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria

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Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria. / Neo, Melissa; Morris, David G; Kuklik, Pawel; Lau, Dennis H; Dimitri, Hany; Lim, Wei-Wen; Sanders, Prashanthan; Saint, David A.

in: CAN J PHYSIOL PHARM, Jahrgang 94, Nr. 5, 05.2016, S. 563-569.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Neo, M, Morris, DG, Kuklik, P, Lau, DH, Dimitri, H, Lim, W-W, Sanders, P & Saint, DA 2016, 'Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria', CAN J PHYSIOL PHARM, Jg. 94, Nr. 5, S. 563-569. https://doi.org/10.1139/cjpp-2015-0194

APA

Neo, M., Morris, D. G., Kuklik, P., Lau, D. H., Dimitri, H., Lim, W-W., Sanders, P., & Saint, D. A. (2016). Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria. CAN J PHYSIOL PHARM, 94(5), 563-569. https://doi.org/10.1139/cjpp-2015-0194

Vancouver

Neo M, Morris DG, Kuklik P, Lau DH, Dimitri H, Lim W-W et al. Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria. CAN J PHYSIOL PHARM. 2016 Mai;94(5):563-569. https://doi.org/10.1139/cjpp-2015-0194

Bibtex

@article{856bbc448d41428bb53229807f22af08,
title = "Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria",
abstract = "We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 × 10 silver chloride electrodes, 0.1 mm diameter and 0.1 mm pitch). A glass microelectrode (100 MΩ) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease. ",
keywords = "Action Potentials, Animals, Arrhythmias, Cardiac/physiopathology, Atrial Function, Electrophysiologic Techniques, Cardiac/instrumentation, Functional Neuroimaging, Heart Atria/innervation, In Vitro Techniques, Intracellular Membranes/physiology, Male, Membrane Potentials, Microarray Analysis, Microelectrodes, Neural Conduction, Pilot Projects, Rats, Sprague-Dawley, Reproducibility of Results, Tachycardia/physiopathology",
author = "Melissa Neo and Morris, {David G} and Pawel Kuklik and Lau, {Dennis H} and Hany Dimitri and Wei-Wen Lim and Prashanthan Sanders and Saint, {David A}",
year = "2016",
month = may,
doi = "10.1139/cjpp-2015-0194",
language = "English",
volume = "94",
pages = "563--569",
journal = "CAN J PHYSIOL PHARM",
issn = "0008-4212",
publisher = "National Research Council of Canada",
number = "5",

}

RIS

TY - JOUR

T1 - Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria

AU - Neo, Melissa

AU - Morris, David G

AU - Kuklik, Pawel

AU - Lau, Dennis H

AU - Dimitri, Hany

AU - Lim, Wei-Wen

AU - Sanders, Prashanthan

AU - Saint, David A

PY - 2016/5

Y1 - 2016/5

N2 - We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 × 10 silver chloride electrodes, 0.1 mm diameter and 0.1 mm pitch). A glass microelectrode (100 MΩ) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease.

AB - We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 × 10 silver chloride electrodes, 0.1 mm diameter and 0.1 mm pitch). A glass microelectrode (100 MΩ) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease.

KW - Action Potentials

KW - Animals

KW - Arrhythmias, Cardiac/physiopathology

KW - Atrial Function

KW - Electrophysiologic Techniques, Cardiac/instrumentation

KW - Functional Neuroimaging

KW - Heart Atria/innervation

KW - In Vitro Techniques

KW - Intracellular Membranes/physiology

KW - Male

KW - Membrane Potentials

KW - Microarray Analysis

KW - Microelectrodes

KW - Neural Conduction

KW - Pilot Projects

KW - Rats, Sprague-Dawley

KW - Reproducibility of Results

KW - Tachycardia/physiopathology

U2 - 10.1139/cjpp-2015-0194

DO - 10.1139/cjpp-2015-0194

M3 - SCORING: Journal article

C2 - 26771118

VL - 94

SP - 563

EP - 569

JO - CAN J PHYSIOL PHARM

JF - CAN J PHYSIOL PHARM

SN - 0008-4212

IS - 5

ER -