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Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells.

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Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells. / Schrepfer, Sonja; Deuse, Tobias; Lange, Claudia; Katzenberg, Regina; Reichenspurner, Hermann; Robbins, Robert C; Pelletier, Marc P.

In: STEM CELLS DEV, Vol. 16, No. 1, 1, 2007, p. 105-107.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schrepfer, S, Deuse, T, Lange, C, Katzenberg, R, Reichenspurner, H, Robbins, RC & Pelletier, MP 2007, 'Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells.', STEM CELLS DEV, vol. 16, no. 1, 1, pp. 105-107. <http://www.ncbi.nlm.nih.gov/pubmed/17348808?dopt=Citation>

APA

Schrepfer, S., Deuse, T., Lange, C., Katzenberg, R., Reichenspurner, H., Robbins, R. C., & Pelletier, M. P. (2007). Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells. STEM CELLS DEV, 16(1), 105-107. [1]. http://www.ncbi.nlm.nih.gov/pubmed/17348808?dopt=Citation

Vancouver

Schrepfer S, Deuse T, Lange C, Katzenberg R, Reichenspurner H, Robbins RC et al. Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells. STEM CELLS DEV. 2007;16(1):105-107. 1.

Bibtex

@article{e87f8203e8124581a47e61249848e8ac,
title = "Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells.",
abstract = "Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.",
author = "Sonja Schrepfer and Tobias Deuse and Claudia Lange and Regina Katzenberg and Hermann Reichenspurner and Robbins, {Robert C} and Pelletier, {Marc P}",
year = "2007",
language = "Deutsch",
volume = "16",
pages = "105--107",
journal = "STEM CELLS DEV",
issn = "1547-3287",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells.

AU - Schrepfer, Sonja

AU - Deuse, Tobias

AU - Lange, Claudia

AU - Katzenberg, Regina

AU - Reichenspurner, Hermann

AU - Robbins, Robert C

AU - Pelletier, Marc P

PY - 2007

Y1 - 2007

N2 - Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.

AB - Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.

M3 - SCORING: Zeitschriftenaufsatz

VL - 16

SP - 105

EP - 107

JO - STEM CELLS DEV

JF - STEM CELLS DEV

SN - 1547-3287

IS - 1

M1 - 1

ER -