Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells.
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Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells. / Schrepfer, Sonja; Deuse, Tobias; Lange, Claudia; Katzenberg, Regina; Reichenspurner, Hermann; Robbins, Robert C; Pelletier, Marc P.
in: STEM CELLS DEV, Jahrgang 16, Nr. 1, 1, 2007, S. 105-107.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells.
AU - Schrepfer, Sonja
AU - Deuse, Tobias
AU - Lange, Claudia
AU - Katzenberg, Regina
AU - Reichenspurner, Hermann
AU - Robbins, Robert C
AU - Pelletier, Marc P
PY - 2007
Y1 - 2007
N2 - Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.
AB - Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.
M3 - SCORING: Zeitschriftenaufsatz
VL - 16
SP - 105
EP - 107
JO - STEM CELLS DEV
JF - STEM CELLS DEV
SN - 1547-3287
IS - 1
M1 - 1
ER -