Silencing Neurons: Tools, Applications, and Experimental Constraints

J Simon Wiegert; Mathias Mahn; Matthias Prigge; Yoav Printz; Ofer Yizhar

doi:10.1016/j.neuron.2017.06.050

Silencing Neurons: Tools, Applications, and Experimental Constraints

Standard

Silencing Neurons: Tools, Applications, and Experimental Constraints. / Wiegert, J Simon; Mahn, Mathias; Prigge, Matthias; Printz, Yoav; Yizhar, Ofer.

In: NEURON, Vol. 95, No. 3, 02.08.2017, p. 504-529.

Research output: SCORING: Contribution to journal › SCORING: Review article › Research

Harvard

Wiegert, JS, Mahn, M, Prigge, M, Printz, Y & Yizhar, O 2017, 'Silencing Neurons: Tools, Applications, and Experimental Constraints', NEURON, vol. 95, no. 3, pp. 504-529. https://doi.org/10.1016/j.neuron.2017.06.050

APA

Wiegert, J. S., Mahn, M., Prigge, M., Printz, Y., & Yizhar, O. (2017). Silencing Neurons: Tools, Applications, and Experimental Constraints. NEURON, 95(3), 504-529. https://doi.org/10.1016/j.neuron.2017.06.050

Vancouver

Wiegert JS, Mahn M, Prigge M, Printz Y, Yizhar O. Silencing Neurons: Tools, Applications, and Experimental Constraints. NEURON. 2017 Aug 2;95(3):504-529. https://doi.org/10.1016/j.neuron.2017.06.050

Bibtex

@article{0217bf38746b4882a8a06f1c011040a6,

title = "Silencing Neurons: Tools, Applications, and Experimental Constraints",

abstract = "Reversible silencing of neuronal activity is a powerful approach for isolating the roles of specific neuronal populations in circuit dynamics and behavior. In contrast with neuronal excitation, for which the majority of studies have used a limited number of optogenetic and chemogenetic tools, the number of genetically encoded tools used for inhibition of neuronal activity has vastly expanded. Silencing strategies vary widely in their mechanism of action and in their spatial and temporal scales. Although such manipulations are commonly applied, the design and interpretation of neuronal silencing experiments present unique challenges, both technically and conceptually. Here, we review the most commonly used tools for silencing neuronal activity and provide an in-depth analysis of their mechanism of action and utility for particular experimental applications. We further discuss the considerations that need to be given to experimental design, analysis, and interpretation of collected data. Finally, we discuss future directions for the development of new silencing approaches in neuroscience.",

keywords = "Journal Article, Review",

author = "Wiegert, {J Simon} and Mathias Mahn and Matthias Prigge and Yoav Printz and Ofer Yizhar",

year = "2017",

month = aug,

day = "2",

doi = "10.1016/j.neuron.2017.06.050",

language = "English",

volume = "95",

pages = "504--529",

journal = "NEURON",

issn = "0896-6273",

publisher = "Cell Press",

number = "3",

}

RIS

TY - JOUR

T1 - Silencing Neurons: Tools, Applications, and Experimental Constraints

AU - Wiegert, J Simon

AU - Mahn, Mathias

AU - Prigge, Matthias

AU - Printz, Yoav

AU - Yizhar, Ofer

PY - 2017/8/2

Y1 - 2017/8/2

N2 - Reversible silencing of neuronal activity is a powerful approach for isolating the roles of specific neuronal populations in circuit dynamics and behavior. In contrast with neuronal excitation, for which the majority of studies have used a limited number of optogenetic and chemogenetic tools, the number of genetically encoded tools used for inhibition of neuronal activity has vastly expanded. Silencing strategies vary widely in their mechanism of action and in their spatial and temporal scales. Although such manipulations are commonly applied, the design and interpretation of neuronal silencing experiments present unique challenges, both technically and conceptually. Here, we review the most commonly used tools for silencing neuronal activity and provide an in-depth analysis of their mechanism of action and utility for particular experimental applications. We further discuss the considerations that need to be given to experimental design, analysis, and interpretation of collected data. Finally, we discuss future directions for the development of new silencing approaches in neuroscience.

AB - Reversible silencing of neuronal activity is a powerful approach for isolating the roles of specific neuronal populations in circuit dynamics and behavior. In contrast with neuronal excitation, for which the majority of studies have used a limited number of optogenetic and chemogenetic tools, the number of genetically encoded tools used for inhibition of neuronal activity has vastly expanded. Silencing strategies vary widely in their mechanism of action and in their spatial and temporal scales. Although such manipulations are commonly applied, the design and interpretation of neuronal silencing experiments present unique challenges, both technically and conceptually. Here, we review the most commonly used tools for silencing neuronal activity and provide an in-depth analysis of their mechanism of action and utility for particular experimental applications. We further discuss the considerations that need to be given to experimental design, analysis, and interpretation of collected data. Finally, we discuss future directions for the development of new silencing approaches in neuroscience.

KW - Journal Article

KW - Review

U2 - 10.1016/j.neuron.2017.06.050

DO - 10.1016/j.neuron.2017.06.050

M3 - SCORING: Review article

C2 - 28772120

VL - 95

SP - 504

EP - 529

JO - NEURON

JF - NEURON

SN - 0896-6273

IS - 3

ER -