Silencing Neurons: Tools, Applications, and Experimental Constraints
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Silencing Neurons: Tools, Applications, and Experimental Constraints. / Wiegert, J Simon; Mahn, Mathias; Prigge, Matthias; Printz, Yoav; Yizhar, Ofer.
in: NEURON, Jahrgang 95, Nr. 3, 02.08.2017, S. 504-529.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Review › Forschung
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TY - JOUR
T1 - Silencing Neurons: Tools, Applications, and Experimental Constraints
AU - Wiegert, J Simon
AU - Mahn, Mathias
AU - Prigge, Matthias
AU - Printz, Yoav
AU - Yizhar, Ofer
N1 - Copyright © 2017 Elsevier Inc. All rights reserved.
PY - 2017/8/2
Y1 - 2017/8/2
N2 - Reversible silencing of neuronal activity is a powerful approach for isolating the roles of specific neuronal populations in circuit dynamics and behavior. In contrast with neuronal excitation, for which the majority of studies have used a limited number of optogenetic and chemogenetic tools, the number of genetically encoded tools used for inhibition of neuronal activity has vastly expanded. Silencing strategies vary widely in their mechanism of action and in their spatial and temporal scales. Although such manipulations are commonly applied, the design and interpretation of neuronal silencing experiments present unique challenges, both technically and conceptually. Here, we review the most commonly used tools for silencing neuronal activity and provide an in-depth analysis of their mechanism of action and utility for particular experimental applications. We further discuss the considerations that need to be given to experimental design, analysis, and interpretation of collected data. Finally, we discuss future directions for the development of new silencing approaches in neuroscience.
AB - Reversible silencing of neuronal activity is a powerful approach for isolating the roles of specific neuronal populations in circuit dynamics and behavior. In contrast with neuronal excitation, for which the majority of studies have used a limited number of optogenetic and chemogenetic tools, the number of genetically encoded tools used for inhibition of neuronal activity has vastly expanded. Silencing strategies vary widely in their mechanism of action and in their spatial and temporal scales. Although such manipulations are commonly applied, the design and interpretation of neuronal silencing experiments present unique challenges, both technically and conceptually. Here, we review the most commonly used tools for silencing neuronal activity and provide an in-depth analysis of their mechanism of action and utility for particular experimental applications. We further discuss the considerations that need to be given to experimental design, analysis, and interpretation of collected data. Finally, we discuss future directions for the development of new silencing approaches in neuroscience.
KW - Journal Article
KW - Review
U2 - 10.1016/j.neuron.2017.06.050
DO - 10.1016/j.neuron.2017.06.050
M3 - SCORING: Review article
C2 - 28772120
VL - 95
SP - 504
EP - 529
JO - NEURON
JF - NEURON
SN - 0896-6273
IS - 3
ER -