Sex-specific epigenetic gene activation profiles are differentially modulated in human placentas affected by intrauterine growth restriction
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Sex-specific epigenetic gene activation profiles are differentially modulated in human placentas affected by intrauterine growth restriction. / Meister, Sarah; Kolben, Thomas; Beyer, Susanne; Hutter, Stefan; Hofmann, Simone; Kuhn, Christina; Messner, Julia; Mayr, Doris; Solano, Maria Emilia; Jegen, Magdalena; Obermeier, Viola; Mahner, Sven; Arck, Petra; Jeschke, Udo.
In: J REPROD IMMUNOL, No. 139, 06.2020, p. 103124.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Sex-specific epigenetic gene activation profiles are differentially modulated in human placentas affected by intrauterine growth restriction
AU - Meister, Sarah
AU - Kolben, Thomas
AU - Beyer, Susanne
AU - Hutter, Stefan
AU - Hofmann, Simone
AU - Kuhn, Christina
AU - Messner, Julia
AU - Mayr, Doris
AU - Solano, Maria Emilia
AU - Jegen, Magdalena
AU - Obermeier, Viola
AU - Mahner, Sven
AU - Arck, Petra
AU - Jeschke, Udo
N1 - Copyright © 2020 Elsevier B.V. All rights reserved.
PY - 2020/6
Y1 - 2020/6
N2 - BACKGROUND: The purpose of this study was to investigate the sex specific expression of histone protein modifications responsible for rapid gene expression in IUGR placentas.PATIENTS AND METHODS: We screened for fetal sex-specific expression of the histone proteins H3K4me3 and H3K9ac in 23 IUGR and 40 control placentas via immunohistochemistry. The trophoblast-like cell line BeWo was used in order to analyze a potential effect of stimulation with prednisolone on H3K4me3 and H3K9ac in vitro. Calculating regression models with additional adjustment for potential confounders were used.RESULTS: A significantly decreased level of H3K4me3 was detectable in female syncytiotrophoblasts, whereas H3K9ac was reduced predominantly in male extravillous throphoblast (EVT). No association to the gestational age existed.CONCLUSION: Our data showed a reduced expression of the histone proteins H3K4me3 (female) and H3K9ac (male) in IUGR, furthermore elevated cortisol levels may lead to a sex-specific down-regulation of histone proteins in IUGR placentas.
AB - BACKGROUND: The purpose of this study was to investigate the sex specific expression of histone protein modifications responsible for rapid gene expression in IUGR placentas.PATIENTS AND METHODS: We screened for fetal sex-specific expression of the histone proteins H3K4me3 and H3K9ac in 23 IUGR and 40 control placentas via immunohistochemistry. The trophoblast-like cell line BeWo was used in order to analyze a potential effect of stimulation with prednisolone on H3K4me3 and H3K9ac in vitro. Calculating regression models with additional adjustment for potential confounders were used.RESULTS: A significantly decreased level of H3K4me3 was detectable in female syncytiotrophoblasts, whereas H3K9ac was reduced predominantly in male extravillous throphoblast (EVT). No association to the gestational age existed.CONCLUSION: Our data showed a reduced expression of the histone proteins H3K4me3 (female) and H3K9ac (male) in IUGR, furthermore elevated cortisol levels may lead to a sex-specific down-regulation of histone proteins in IUGR placentas.
U2 - 10.1016/j.jri.2020.103124
DO - 10.1016/j.jri.2020.103124
M3 - SCORING: Journal article
C2 - 32289580
SP - 103124
JO - J REPROD IMMUNOL
JF - J REPROD IMMUNOL
SN - 0165-0378
IS - 139
ER -