Scleral Inflammation around Collector Channels in Eyes with Primary Open-Angle Glaucoma
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Scleral Inflammation around Collector Channels in Eyes with Primary Open-Angle Glaucoma. / Schultheiss, Maximilian; Voykov, Bogomil; Klemm, Maren; Gross, Ulrich; Schultheiss, Heinz-Peter; Spitzer, Martin S; Casagrande, Maria.
In: OCUL IMMUNOL INFLAMM, 2020, p. 1-7.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Scleral Inflammation around Collector Channels in Eyes with Primary Open-Angle Glaucoma
AU - Schultheiss, Maximilian
AU - Voykov, Bogomil
AU - Klemm, Maren
AU - Gross, Ulrich
AU - Schultheiss, Heinz-Peter
AU - Spitzer, Martin S
AU - Casagrande, Maria
PY - 2020
Y1 - 2020
N2 - Purpose: Investigating the existence of inflammation in the distal outflow system posterior of the Schlemm's canal in primary open-angle glaucoma (POAG).Methods: Scleral biopsies (n = 62) from POAG-patients were taken during deep sclerectomy and fixed either in formalin or RNAlater®. Histologic (hematoxylin & eosin) and immunohistological staining for CD 3 and CD 45RO were performed.Results: Cellular infiltration of immunocompetent cells (CD 3 and CD 45RO positive cells) exists around collector channels (CC). This inflammation is limited to the area around the CCs. Ninety-two percent of the biopsies are positive for inflammation. Untreated, dysgenetic glaucoma eyes and 8% of POAG eyes were negative for inflammation. Neither the use of benzalkonium chloride nor the number and type of preoperative antiglaucomatous medication correlated to the immunohistological result.Conclusion: In POAG a diverse cellular infiltration exists around the CCs in the vast majority of biopsies. This could have major diagnostic and therapeutic consequences for the treatment of POAG.Abbreviations: POAG: primary open-angle glaucoma; CC: collector channel; AH: aqueous humor; TM: trabecular meshwork; SC: Schlemm's canal; HE: hematoxylin & eosin; APC: antigen-presenting cell.
AB - Purpose: Investigating the existence of inflammation in the distal outflow system posterior of the Schlemm's canal in primary open-angle glaucoma (POAG).Methods: Scleral biopsies (n = 62) from POAG-patients were taken during deep sclerectomy and fixed either in formalin or RNAlater®. Histologic (hematoxylin & eosin) and immunohistological staining for CD 3 and CD 45RO were performed.Results: Cellular infiltration of immunocompetent cells (CD 3 and CD 45RO positive cells) exists around collector channels (CC). This inflammation is limited to the area around the CCs. Ninety-two percent of the biopsies are positive for inflammation. Untreated, dysgenetic glaucoma eyes and 8% of POAG eyes were negative for inflammation. Neither the use of benzalkonium chloride nor the number and type of preoperative antiglaucomatous medication correlated to the immunohistological result.Conclusion: In POAG a diverse cellular infiltration exists around the CCs in the vast majority of biopsies. This could have major diagnostic and therapeutic consequences for the treatment of POAG.Abbreviations: POAG: primary open-angle glaucoma; CC: collector channel; AH: aqueous humor; TM: trabecular meshwork; SC: Schlemm's canal; HE: hematoxylin & eosin; APC: antigen-presenting cell.
U2 - 10.1080/09273948.2020.1746355
DO - 10.1080/09273948.2020.1746355
M3 - SCORING: Journal article
C2 - 32400241
SP - 1
EP - 7
JO - OCUL IMMUNOL INFLAMM
JF - OCUL IMMUNOL INFLAMM
SN - 0927-3948
ER -