Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish
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Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish. / Geudens, Ilse; Herpers, Robert; Hermans, Karlien; Segura, Inmaculada; Ruiz de Almodovar, Carmen; Bussmann, Jeroen; De Smet, Frederik; Vandevelde, Wouter; Hogan, Benjamin M; Siekmann, Arndt; Claes, Filip; Moore, John C; Pistocchi, Anna Silvia; Loges, Sonja; Mazzone, Massimiliano; Mariggi, Giovanni; Bruyère, Françoise; Cotelli, Franco; Kerjaschki, Dontscho; Noël, Agnes; Foidart, Jean-Michel; Gerhardt, Holger; Ny, Annelii; Langenberg, Tobias; Lawson, Nathan D; Duckers, Henricus J; Schulte-Merker, Stefan; Carmeliet, Peter; Dewerchin, Mieke.
In: ARTERIOSCL THROM VAS, Vol. 30, No. 9, 09.2010, p. 1695-702.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish
AU - Geudens, Ilse
AU - Herpers, Robert
AU - Hermans, Karlien
AU - Segura, Inmaculada
AU - Ruiz de Almodovar, Carmen
AU - Bussmann, Jeroen
AU - De Smet, Frederik
AU - Vandevelde, Wouter
AU - Hogan, Benjamin M
AU - Siekmann, Arndt
AU - Claes, Filip
AU - Moore, John C
AU - Pistocchi, Anna Silvia
AU - Loges, Sonja
AU - Mazzone, Massimiliano
AU - Mariggi, Giovanni
AU - Bruyère, Françoise
AU - Cotelli, Franco
AU - Kerjaschki, Dontscho
AU - Noël, Agnes
AU - Foidart, Jean-Michel
AU - Gerhardt, Holger
AU - Ny, Annelii
AU - Langenberg, Tobias
AU - Lawson, Nathan D
AU - Duckers, Henricus J
AU - Schulte-Merker, Stefan
AU - Carmeliet, Peter
AU - Dewerchin, Mieke
PY - 2010/9
Y1 - 2010/9
N2 - OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development.METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates.CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.
AB - OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development.METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates.CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.
KW - Animals
KW - Animals, Genetically Modified
KW - Biomarkers
KW - COS Cells
KW - Cell Movement
KW - Cell Proliferation
KW - Cercopithecus aethiops
KW - Coculture Techniques
KW - Embryo, Nonmammalian
KW - Endothelial Cells
KW - Gene Expression Regulation, Developmental
KW - Gene Knockdown Techniques
KW - Gene Silencing
KW - Humans
KW - Intracellular Signaling Peptides and Proteins
KW - Luminescent Proteins
KW - Lymphangiogenesis
KW - Lymphatic System
KW - Membrane Proteins
KW - RNA, Messenger
KW - Receptors, Notch
KW - Signal Transduction
KW - Thoracic Duct
KW - Zebrafish
KW - Zebrafish Proteins
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1161/ATVBAHA.110.203034
DO - 10.1161/ATVBAHA.110.203034
M3 - SCORING: Journal article
C2 - 20466977
VL - 30
SP - 1695
EP - 1702
JO - ARTERIOSCL THROM VAS
JF - ARTERIOSCL THROM VAS
SN - 1079-5642
IS - 9
ER -