[Replication of ColE 1-related plasmids at increased growth temperature depending on rom function].
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[Replication of ColE 1-related plasmids at increased growth temperature depending on rom function]. / Riethdorf, Sabine; Ulrich, A; Hecker, M.
In: Z NATURFORSCH C, Vol. 46, No. 11-12, 11-12, 1991, p. 1063-1066.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - [Replication of ColE 1-related plasmids at increased growth temperature depending on rom function].
AU - Riethdorf, Sabine
AU - Ulrich, A
AU - Hecker, M
PY - 1991
Y1 - 1991
N2 - The content of ColE 1-related plasmids increased about 4-6-fold after a temperature-shift from 30 to 42 degrees C (45 degrees C) if the rom-region of the plasmids was deleted. The copy number of rom(+)-plasmids did not change after the temperature shift. All rom(-)-plasmids tested in this study showed this plasmid amplification. The Rom-protein is capable of inhibiting plasmid replication by stabilization the initial reversible stage of the association of RNA I with the primer precursor RNA II. We suggest that the temperature-dependent enhancement of the copy number of rom(-)-plasmids is due to a destabilization of this initial phase of the RNA I-preprimer interaction at high temperatures which is suppressed by the Rom-protein in cells with rom(+)-plasmids.
AB - The content of ColE 1-related plasmids increased about 4-6-fold after a temperature-shift from 30 to 42 degrees C (45 degrees C) if the rom-region of the plasmids was deleted. The copy number of rom(+)-plasmids did not change after the temperature shift. All rom(-)-plasmids tested in this study showed this plasmid amplification. The Rom-protein is capable of inhibiting plasmid replication by stabilization the initial reversible stage of the association of RNA I with the primer precursor RNA II. We suggest that the temperature-dependent enhancement of the copy number of rom(-)-plasmids is due to a destabilization of this initial phase of the RNA I-preprimer interaction at high temperatures which is suppressed by the Rom-protein in cells with rom(+)-plasmids.
KW - Kinetics
KW - Temperature
KW - Bacterial Proteins/genetics/metabolism
KW - Bacteriocin Plasmids
KW - Escherichia coli/genetics/metabolism
KW - RNA Precursors/metabolism
KW - Kinetics
KW - Temperature
KW - Bacterial Proteins/genetics/metabolism
KW - Bacteriocin Plasmids
KW - Escherichia coli/genetics/metabolism
KW - RNA Precursors/metabolism
M3 - SCORING: Zeitschriftenaufsatz
VL - 46
SP - 1063
EP - 1066
JO - Z NATURFORSCH C
JF - Z NATURFORSCH C
SN - 0939-5075
IS - 11-12
M1 - 11-12
ER -