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[Replication of ColE 1-related plasmids at increased growth temperature depending on rom function].

Standard

[Replication of ColE 1-related plasmids at increased growth temperature depending on rom function]. / Riethdorf, Sabine; Ulrich, A; Hecker, M.

in: Z NATURFORSCH C, Jahrgang 46, Nr. 11-12, 11-12, 1991, S. 1063-1066.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Riethdorf, S, Ulrich, A & Hecker, M 1991, '[Replication of ColE 1-related plasmids at increased growth temperature depending on rom function].', Z NATURFORSCH C, Jg. 46, Nr. 11-12, 11-12, S. 1063-1066. <http://www.ncbi.nlm.nih.gov/pubmed/1817515?dopt=Citation>

APA

Riethdorf, S., Ulrich, A., & Hecker, M. (1991). [Replication of ColE 1-related plasmids at increased growth temperature depending on rom function]. Z NATURFORSCH C, 46(11-12), 1063-1066. [11-12]. http://www.ncbi.nlm.nih.gov/pubmed/1817515?dopt=Citation

Vancouver

Riethdorf S, Ulrich A, Hecker M. [Replication of ColE 1-related plasmids at increased growth temperature depending on rom function]. Z NATURFORSCH C. 1991;46(11-12):1063-1066. 11-12.

Bibtex

@article{7e92aa5fc280441dbe1af9990fe6c465,
title = "[Replication of ColE 1-related plasmids at increased growth temperature depending on rom function].",
abstract = "The content of ColE 1-related plasmids increased about 4-6-fold after a temperature-shift from 30 to 42 degrees C (45 degrees C) if the rom-region of the plasmids was deleted. The copy number of rom(+)-plasmids did not change after the temperature shift. All rom(-)-plasmids tested in this study showed this plasmid amplification. The Rom-protein is capable of inhibiting plasmid replication by stabilization the initial reversible stage of the association of RNA I with the primer precursor RNA II. We suggest that the temperature-dependent enhancement of the copy number of rom(-)-plasmids is due to a destabilization of this initial phase of the RNA I-preprimer interaction at high temperatures which is suppressed by the Rom-protein in cells with rom(+)-plasmids.",
keywords = "Kinetics, Temperature, Bacterial Proteins/genetics/*metabolism, *Bacteriocin Plasmids, Escherichia coli/*genetics/metabolism, RNA Precursors/metabolism, Kinetics, Temperature, Bacterial Proteins/genetics/*metabolism, *Bacteriocin Plasmids, Escherichia coli/*genetics/metabolism, RNA Precursors/metabolism",
author = "Sabine Riethdorf and A Ulrich and M Hecker",
year = "1991",
language = "Deutsch",
volume = "46",
pages = "1063--1066",
journal = "Z NATURFORSCH C",
issn = "0939-5075",
publisher = "Verlag der Zeitschrift fur Naturforschung",
number = "11-12",

}

RIS

TY - JOUR

T1 - [Replication of ColE 1-related plasmids at increased growth temperature depending on rom function].

AU - Riethdorf, Sabine

AU - Ulrich, A

AU - Hecker, M

PY - 1991

Y1 - 1991

N2 - The content of ColE 1-related plasmids increased about 4-6-fold after a temperature-shift from 30 to 42 degrees C (45 degrees C) if the rom-region of the plasmids was deleted. The copy number of rom(+)-plasmids did not change after the temperature shift. All rom(-)-plasmids tested in this study showed this plasmid amplification. The Rom-protein is capable of inhibiting plasmid replication by stabilization the initial reversible stage of the association of RNA I with the primer precursor RNA II. We suggest that the temperature-dependent enhancement of the copy number of rom(-)-plasmids is due to a destabilization of this initial phase of the RNA I-preprimer interaction at high temperatures which is suppressed by the Rom-protein in cells with rom(+)-plasmids.

AB - The content of ColE 1-related plasmids increased about 4-6-fold after a temperature-shift from 30 to 42 degrees C (45 degrees C) if the rom-region of the plasmids was deleted. The copy number of rom(+)-plasmids did not change after the temperature shift. All rom(-)-plasmids tested in this study showed this plasmid amplification. The Rom-protein is capable of inhibiting plasmid replication by stabilization the initial reversible stage of the association of RNA I with the primer precursor RNA II. We suggest that the temperature-dependent enhancement of the copy number of rom(-)-plasmids is due to a destabilization of this initial phase of the RNA I-preprimer interaction at high temperatures which is suppressed by the Rom-protein in cells with rom(+)-plasmids.

KW - Kinetics

KW - Temperature

KW - Bacterial Proteins/genetics/metabolism

KW - Bacteriocin Plasmids

KW - Escherichia coli/genetics/metabolism

KW - RNA Precursors/metabolism

KW - Kinetics

KW - Temperature

KW - Bacterial Proteins/genetics/metabolism

KW - Bacteriocin Plasmids

KW - Escherichia coli/genetics/metabolism

KW - RNA Precursors/metabolism

M3 - SCORING: Zeitschriftenaufsatz

VL - 46

SP - 1063

EP - 1066

JO - Z NATURFORSCH C

JF - Z NATURFORSCH C

SN - 0939-5075

IS - 11-12

M1 - 11-12

ER -