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Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer

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Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer. / Schütze, Dina; Milde-Langosch, Karin; Witzel, Isabell; Rody, Achim; Karn, Thomas; Schmidt, Marcus; Choschzick, Matthias; Jänicke, Fritz; Müller, Volkmar.

In: J CANCER RES CLIN, Vol. 139, No. 5, 01.05.2013, p. 747-54.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schütze, D, Milde-Langosch, K, Witzel, I, Rody, A, Karn, T, Schmidt, M, Choschzick, M, Jänicke, F & Müller, V 2013, 'Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer', J CANCER RES CLIN, vol. 139, no. 5, pp. 747-54. https://doi.org/10.1007/s00432-013-1378-4

APA

Schütze, D., Milde-Langosch, K., Witzel, I., Rody, A., Karn, T., Schmidt, M., Choschzick, M., Jänicke, F., & Müller, V. (2013). Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer. J CANCER RES CLIN, 139(5), 747-54. https://doi.org/10.1007/s00432-013-1378-4

Vancouver

Schütze D, Milde-Langosch K, Witzel I, Rody A, Karn T, Schmidt M et al. Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer. J CANCER RES CLIN. 2013 May 1;139(5):747-54. https://doi.org/10.1007/s00432-013-1378-4

Bibtex

@article{d802a11d5bc54578a5f41fca5da5fdc6,
title = "Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer",
abstract = "BACKGROUND: Carbonic anhydrase IX (CAIX) is involved in pH homeostasis, growth and survival of tumor cells. Besides the membranous form of CAIX, a soluble form is detectable in serum (s-CAIX). Overexpression of CAIX in tumors offers the opportunity for therapeutic strategies such as CAIX targeting antibodies. The aim of this study was to examine the relationships of CAIX mRNA expression and s-CAIX levels with clinicopathological parameters and survival of patients with primary breast cancer.METHODS: Tumor tissue of 169 primary breast cancer patients was analyzed for RNA expression by microarray analysis (Affymetrix HG-U133A). Concentration of s-CAIX was determined by ELISA in blood samples of 140 patients.RESULTS: In tumor tissue, CAIX mRNA signal intensities (MAS5 values) ranged from 34 to 2,513. Higher CAIX expression was associated with younger age (CONCLUSION: In contrast to reported immunohistochemical studies, we performed RNA-based tissue analyses of CAIX expression and, for the first time, analyzed CAIX serum levels in primary breast cancer. The correlations between CAIX RNA expression and prognostic factors and patient outcome support a biologic role of CAIX in early breast cancer. A role of s-CAIX in primary breast cancer is not supported by our findings.",
keywords = "Adult, Aged, Aged, 80 and over, Antigens, Neoplasm, Breast Neoplasms, Carbonic Anhydrases, Female, Gene Expression, Humans, Middle Aged, Neoplasm Staging, Prognosis, Tumor Markers, Biological",
author = "Dina Sch{\"u}tze and Karin Milde-Langosch and Isabell Witzel and Achim Rody and Thomas Karn and Marcus Schmidt and Matthias Choschzick and Fritz J{\"a}nicke and Volkmar M{\"u}ller",
year = "2013",
month = may,
day = "1",
doi = "10.1007/s00432-013-1378-4",
language = "English",
volume = "139",
pages = "747--54",
journal = "J CANCER RES CLIN",
issn = "0171-5216",
publisher = "Springer",
number = "5",

}

RIS

TY - JOUR

T1 - Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer

AU - Schütze, Dina

AU - Milde-Langosch, Karin

AU - Witzel, Isabell

AU - Rody, Achim

AU - Karn, Thomas

AU - Schmidt, Marcus

AU - Choschzick, Matthias

AU - Jänicke, Fritz

AU - Müller, Volkmar

PY - 2013/5/1

Y1 - 2013/5/1

N2 - BACKGROUND: Carbonic anhydrase IX (CAIX) is involved in pH homeostasis, growth and survival of tumor cells. Besides the membranous form of CAIX, a soluble form is detectable in serum (s-CAIX). Overexpression of CAIX in tumors offers the opportunity for therapeutic strategies such as CAIX targeting antibodies. The aim of this study was to examine the relationships of CAIX mRNA expression and s-CAIX levels with clinicopathological parameters and survival of patients with primary breast cancer.METHODS: Tumor tissue of 169 primary breast cancer patients was analyzed for RNA expression by microarray analysis (Affymetrix HG-U133A). Concentration of s-CAIX was determined by ELISA in blood samples of 140 patients.RESULTS: In tumor tissue, CAIX mRNA signal intensities (MAS5 values) ranged from 34 to 2,513. Higher CAIX expression was associated with younger age (CONCLUSION: In contrast to reported immunohistochemical studies, we performed RNA-based tissue analyses of CAIX expression and, for the first time, analyzed CAIX serum levels in primary breast cancer. The correlations between CAIX RNA expression and prognostic factors and patient outcome support a biologic role of CAIX in early breast cancer. A role of s-CAIX in primary breast cancer is not supported by our findings.

AB - BACKGROUND: Carbonic anhydrase IX (CAIX) is involved in pH homeostasis, growth and survival of tumor cells. Besides the membranous form of CAIX, a soluble form is detectable in serum (s-CAIX). Overexpression of CAIX in tumors offers the opportunity for therapeutic strategies such as CAIX targeting antibodies. The aim of this study was to examine the relationships of CAIX mRNA expression and s-CAIX levels with clinicopathological parameters and survival of patients with primary breast cancer.METHODS: Tumor tissue of 169 primary breast cancer patients was analyzed for RNA expression by microarray analysis (Affymetrix HG-U133A). Concentration of s-CAIX was determined by ELISA in blood samples of 140 patients.RESULTS: In tumor tissue, CAIX mRNA signal intensities (MAS5 values) ranged from 34 to 2,513. Higher CAIX expression was associated with younger age (CONCLUSION: In contrast to reported immunohistochemical studies, we performed RNA-based tissue analyses of CAIX expression and, for the first time, analyzed CAIX serum levels in primary breast cancer. The correlations between CAIX RNA expression and prognostic factors and patient outcome support a biologic role of CAIX in early breast cancer. A role of s-CAIX in primary breast cancer is not supported by our findings.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Antigens, Neoplasm

KW - Breast Neoplasms

KW - Carbonic Anhydrases

KW - Female

KW - Gene Expression

KW - Humans

KW - Middle Aged

KW - Neoplasm Staging

KW - Prognosis

KW - Tumor Markers, Biological

U2 - 10.1007/s00432-013-1378-4

DO - 10.1007/s00432-013-1378-4

M3 - SCORING: Journal article

C2 - 23358720

VL - 139

SP - 747

EP - 754

JO - J CANCER RES CLIN

JF - J CANCER RES CLIN

SN - 0171-5216

IS - 5

ER -