Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer
Standard
Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer. / Schütze, Dina; Milde-Langosch, Karin; Witzel, Isabell; Rody, Achim; Karn, Thomas; Schmidt, Marcus; Choschzick, Matthias; Jänicke, Fritz; Müller, Volkmar.
in: J CANCER RES CLIN, Jahrgang 139, Nr. 5, 01.05.2013, S. 747-54.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer
AU - Schütze, Dina
AU - Milde-Langosch, Karin
AU - Witzel, Isabell
AU - Rody, Achim
AU - Karn, Thomas
AU - Schmidt, Marcus
AU - Choschzick, Matthias
AU - Jänicke, Fritz
AU - Müller, Volkmar
PY - 2013/5/1
Y1 - 2013/5/1
N2 - BACKGROUND: Carbonic anhydrase IX (CAIX) is involved in pH homeostasis, growth and survival of tumor cells. Besides the membranous form of CAIX, a soluble form is detectable in serum (s-CAIX). Overexpression of CAIX in tumors offers the opportunity for therapeutic strategies such as CAIX targeting antibodies. The aim of this study was to examine the relationships of CAIX mRNA expression and s-CAIX levels with clinicopathological parameters and survival of patients with primary breast cancer.METHODS: Tumor tissue of 169 primary breast cancer patients was analyzed for RNA expression by microarray analysis (Affymetrix HG-U133A). Concentration of s-CAIX was determined by ELISA in blood samples of 140 patients.RESULTS: In tumor tissue, CAIX mRNA signal intensities (MAS5 values) ranged from 34 to 2,513. Higher CAIX expression was associated with younger age (CONCLUSION: In contrast to reported immunohistochemical studies, we performed RNA-based tissue analyses of CAIX expression and, for the first time, analyzed CAIX serum levels in primary breast cancer. The correlations between CAIX RNA expression and prognostic factors and patient outcome support a biologic role of CAIX in early breast cancer. A role of s-CAIX in primary breast cancer is not supported by our findings.
AB - BACKGROUND: Carbonic anhydrase IX (CAIX) is involved in pH homeostasis, growth and survival of tumor cells. Besides the membranous form of CAIX, a soluble form is detectable in serum (s-CAIX). Overexpression of CAIX in tumors offers the opportunity for therapeutic strategies such as CAIX targeting antibodies. The aim of this study was to examine the relationships of CAIX mRNA expression and s-CAIX levels with clinicopathological parameters and survival of patients with primary breast cancer.METHODS: Tumor tissue of 169 primary breast cancer patients was analyzed for RNA expression by microarray analysis (Affymetrix HG-U133A). Concentration of s-CAIX was determined by ELISA in blood samples of 140 patients.RESULTS: In tumor tissue, CAIX mRNA signal intensities (MAS5 values) ranged from 34 to 2,513. Higher CAIX expression was associated with younger age (CONCLUSION: In contrast to reported immunohistochemical studies, we performed RNA-based tissue analyses of CAIX expression and, for the first time, analyzed CAIX serum levels in primary breast cancer. The correlations between CAIX RNA expression and prognostic factors and patient outcome support a biologic role of CAIX in early breast cancer. A role of s-CAIX in primary breast cancer is not supported by our findings.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Antigens, Neoplasm
KW - Breast Neoplasms
KW - Carbonic Anhydrases
KW - Female
KW - Gene Expression
KW - Humans
KW - Middle Aged
KW - Neoplasm Staging
KW - Prognosis
KW - Tumor Markers, Biological
U2 - 10.1007/s00432-013-1378-4
DO - 10.1007/s00432-013-1378-4
M3 - SCORING: Journal article
C2 - 23358720
VL - 139
SP - 747
EP - 754
JO - J CANCER RES CLIN
JF - J CANCER RES CLIN
SN - 0171-5216
IS - 5
ER -