Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample
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Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample. / Günther, Thomas; Haas, Ludwig; Alawi, Malik; Wohlsein, Peter; Marks, Jerzy; Grundhoff, Adam; Becher, Paul; Fischer, Nicole.
In: SCI REP-UK, Vol. 7, No. 1, 16.06.2017, p. 3734.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample
AU - Günther, Thomas
AU - Haas, Ludwig
AU - Alawi, Malik
AU - Wohlsein, Peter
AU - Marks, Jerzy
AU - Grundhoff, Adam
AU - Becher, Paul
AU - Fischer, Nicole
PY - 2017/6/16
Y1 - 2017/6/16
N2 - We recovered the first full-length poxvirus genome, including the terminal hairpin region, directly from complex clinical material using a combination of second generation short read and third generation nanopore sequencing technologies. The complete viral genome sequence was directly recovered from a skin lesion of a grey seal thereby preventing sequence changes due to in vitro passaging of the virus. Subsequent analysis of the proteins encoded by this virus identified genes specific for skin adaptation and pathogenesis of parapoxviruses. These data warrant the classification of seal parapoxvirus, tentatively designated SePPV, as a new species within the genus Parapoxvirus.
AB - We recovered the first full-length poxvirus genome, including the terminal hairpin region, directly from complex clinical material using a combination of second generation short read and third generation nanopore sequencing technologies. The complete viral genome sequence was directly recovered from a skin lesion of a grey seal thereby preventing sequence changes due to in vitro passaging of the virus. Subsequent analysis of the proteins encoded by this virus identified genes specific for skin adaptation and pathogenesis of parapoxviruses. These data warrant the classification of seal parapoxvirus, tentatively designated SePPV, as a new species within the genus Parapoxvirus.
KW - Journal Article
U2 - 10.1038/s41598-017-03997-y
DO - 10.1038/s41598-017-03997-y
M3 - SCORING: Journal article
C2 - 28623312
VL - 7
SP - 3734
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
IS - 1
ER -