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Real-time investigation of dynamic protein crystallization in living cells

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Real-time investigation of dynamic protein crystallization in living cells. / Schönherr, R; Klinge, M; Rudolph, J M; Fita, K; Rehders, D; Lübber, F; Schneegans, S; Majoul, I V; Duszenko, M; Betzel, C; Brandariz-Nuñez, A; Martinez-Costas, J; Duden, R; Redecke, L.

In: STRUCT DYNAM-US, Vol. 2, No. 4, 07.2015, p. 041712.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schönherr, R, Klinge, M, Rudolph, JM, Fita, K, Rehders, D, Lübber, F, Schneegans, S, Majoul, IV, Duszenko, M, Betzel, C, Brandariz-Nuñez, A, Martinez-Costas, J, Duden, R & Redecke, L 2015, 'Real-time investigation of dynamic protein crystallization in living cells', STRUCT DYNAM-US, vol. 2, no. 4, pp. 041712. https://doi.org/10.1063/1.4921591

APA

Schönherr, R., Klinge, M., Rudolph, J. M., Fita, K., Rehders, D., Lübber, F., Schneegans, S., Majoul, I. V., Duszenko, M., Betzel, C., Brandariz-Nuñez, A., Martinez-Costas, J., Duden, R., & Redecke, L. (2015). Real-time investigation of dynamic protein crystallization in living cells. STRUCT DYNAM-US, 2(4), 041712. https://doi.org/10.1063/1.4921591

Vancouver

Schönherr R, Klinge M, Rudolph JM, Fita K, Rehders D, Lübber F et al. Real-time investigation of dynamic protein crystallization in living cells. STRUCT DYNAM-US. 2015 Jul;2(4):041712. https://doi.org/10.1063/1.4921591

Bibtex

@article{84b30a1f4b014498b79dd08cafa5beb9,
title = "Real-time investigation of dynamic protein crystallization in living cells",
abstract = "X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro. ",
author = "R Sch{\"o}nherr and M Klinge and Rudolph, {J M} and K Fita and D Rehders and F L{\"u}bber and S Schneegans and Majoul, {I V} and M Duszenko and C Betzel and A Brandariz-Nu{\~n}ez and J Martinez-Costas and R Duden and L Redecke",
year = "2015",
month = jul,
doi = "10.1063/1.4921591",
language = "English",
volume = "2",
pages = "041712",
journal = "STRUCT DYNAM-US",
issn = "2329-7778",
publisher = "AAPM - American Association of Physicists in Medicine",
number = "4",

}

RIS

TY - JOUR

T1 - Real-time investigation of dynamic protein crystallization in living cells

AU - Schönherr, R

AU - Klinge, M

AU - Rudolph, J M

AU - Fita, K

AU - Rehders, D

AU - Lübber, F

AU - Schneegans, S

AU - Majoul, I V

AU - Duszenko, M

AU - Betzel, C

AU - Brandariz-Nuñez, A

AU - Martinez-Costas, J

AU - Duden, R

AU - Redecke, L

PY - 2015/7

Y1 - 2015/7

N2 - X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.

AB - X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.

U2 - 10.1063/1.4921591

DO - 10.1063/1.4921591

M3 - SCORING: Journal article

C2 - 26798811

VL - 2

SP - 041712

JO - STRUCT DYNAM-US

JF - STRUCT DYNAM-US

SN - 2329-7778

IS - 4

ER -