Real-time investigation of dynamic protein crystallization in living cells
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Real-time investigation of dynamic protein crystallization in living cells. / Schönherr, R; Klinge, M; Rudolph, J M; Fita, K; Rehders, D; Lübber, F; Schneegans, S; Majoul, I V; Duszenko, M; Betzel, C; Brandariz-Nuñez, A; Martinez-Costas, J; Duden, R; Redecke, L.
in: STRUCT DYNAM-US, Jahrgang 2, Nr. 4, 07.2015, S. 041712.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Real-time investigation of dynamic protein crystallization in living cells
AU - Schönherr, R
AU - Klinge, M
AU - Rudolph, J M
AU - Fita, K
AU - Rehders, D
AU - Lübber, F
AU - Schneegans, S
AU - Majoul, I V
AU - Duszenko, M
AU - Betzel, C
AU - Brandariz-Nuñez, A
AU - Martinez-Costas, J
AU - Duden, R
AU - Redecke, L
PY - 2015/7
Y1 - 2015/7
N2 - X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.
AB - X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.
U2 - 10.1063/1.4921591
DO - 10.1063/1.4921591
M3 - SCORING: Journal article
C2 - 26798811
VL - 2
SP - 041712
JO - STRUCT DYNAM-US
JF - STRUCT DYNAM-US
SN - 2329-7778
IS - 4
ER -