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Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive

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Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive. / Zielinski, Kara A; Prester, Andreas; Andaleeb, Hina; Bui, Soi; Yefanov, Oleksandr; Catapano, Lucrezia; Henkel, Alessandra; Wiedorn, Max O; Lorbeer, Olga; Crosas, Eva; Meyer, Jan; Mariani, Valerio; Domaracky, Martin; White, Thomas A; Fleckenstein, Holger; Sarrou, Iosifina; Werner, Nadine; Betzel, Christian; Rohde, Holger; Aepfelbacher, Martin; Chapman, Henry N; Perbandt, Markus; Steiner, Roberto A; Oberthuer, Dominik.

In: IUCRJ, Vol. 9, No. Pt 6, 01.11.2022, p. 778-791.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Zielinski, KA, Prester, A, Andaleeb, H, Bui, S, Yefanov, O, Catapano, L, Henkel, A, Wiedorn, MO, Lorbeer, O, Crosas, E, Meyer, J, Mariani, V, Domaracky, M, White, TA, Fleckenstein, H, Sarrou, I, Werner, N, Betzel, C, Rohde, H, Aepfelbacher, M, Chapman, HN, Perbandt, M, Steiner, RA & Oberthuer, D 2022, 'Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive', IUCRJ, vol. 9, no. Pt 6, pp. 778-791. https://doi.org/10.1107/S2052252522010193

APA

Zielinski, K. A., Prester, A., Andaleeb, H., Bui, S., Yefanov, O., Catapano, L., Henkel, A., Wiedorn, M. O., Lorbeer, O., Crosas, E., Meyer, J., Mariani, V., Domaracky, M., White, T. A., Fleckenstein, H., Sarrou, I., Werner, N., Betzel, C., Rohde, H., ... Oberthuer, D. (2022). Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive. IUCRJ, 9(Pt 6), 778-791. https://doi.org/10.1107/S2052252522010193

Vancouver

Zielinski KA, Prester A, Andaleeb H, Bui S, Yefanov O, Catapano L et al. Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive. IUCRJ. 2022 Nov 1;9(Pt 6):778-791. https://doi.org/10.1107/S2052252522010193

Bibtex

@article{f418687e44cc4fc7844b3f9e11146950,
title = "Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive",
abstract = "Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance (Klebsiella pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.",
author = "Zielinski, {Kara A} and Andreas Prester and Hina Andaleeb and Soi Bui and Oleksandr Yefanov and Lucrezia Catapano and Alessandra Henkel and Wiedorn, {Max O} and Olga Lorbeer and Eva Crosas and Jan Meyer and Valerio Mariani and Martin Domaracky and White, {Thomas A} and Holger Fleckenstein and Iosifina Sarrou and Nadine Werner and Christian Betzel and Holger Rohde and Martin Aepfelbacher and Chapman, {Henry N} and Markus Perbandt and Steiner, {Roberto A} and Dominik Oberthuer",
note = "{\textcopyright} Kara A Zielinski et al. 2022.",
year = "2022",
month = nov,
day = "1",
doi = "10.1107/S2052252522010193",
language = "English",
volume = "9",
pages = "778--791",
journal = "IUCRJ",
issn = "2052-2525",
publisher = "International Union of Crystallography",
number = "Pt 6",

}

RIS

TY - JOUR

T1 - Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive

AU - Zielinski, Kara A

AU - Prester, Andreas

AU - Andaleeb, Hina

AU - Bui, Soi

AU - Yefanov, Oleksandr

AU - Catapano, Lucrezia

AU - Henkel, Alessandra

AU - Wiedorn, Max O

AU - Lorbeer, Olga

AU - Crosas, Eva

AU - Meyer, Jan

AU - Mariani, Valerio

AU - Domaracky, Martin

AU - White, Thomas A

AU - Fleckenstein, Holger

AU - Sarrou, Iosifina

AU - Werner, Nadine

AU - Betzel, Christian

AU - Rohde, Holger

AU - Aepfelbacher, Martin

AU - Chapman, Henry N

AU - Perbandt, Markus

AU - Steiner, Roberto A

AU - Oberthuer, Dominik

N1 - © Kara A Zielinski et al. 2022.

PY - 2022/11/1

Y1 - 2022/11/1

N2 - Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance (Klebsiella pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.

AB - Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance (Klebsiella pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.

U2 - 10.1107/S2052252522010193

DO - 10.1107/S2052252522010193

M3 - SCORING: Journal article

C2 - 36381150

VL - 9

SP - 778

EP - 791

JO - IUCRJ

JF - IUCRJ

SN - 2052-2525

IS - Pt 6

ER -