Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive
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Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive. / Zielinski, Kara A; Prester, Andreas; Andaleeb, Hina; Bui, Soi; Yefanov, Oleksandr; Catapano, Lucrezia; Henkel, Alessandra; Wiedorn, Max O; Lorbeer, Olga; Crosas, Eva; Meyer, Jan; Mariani, Valerio; Domaracky, Martin; White, Thomas A; Fleckenstein, Holger; Sarrou, Iosifina; Werner, Nadine; Betzel, Christian; Rohde, Holger; Aepfelbacher, Martin; Chapman, Henry N; Perbandt, Markus; Steiner, Roberto A; Oberthuer, Dominik.
in: IUCRJ, Jahrgang 9, Nr. Pt 6, 01.11.2022, S. 778-791.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive
AU - Zielinski, Kara A
AU - Prester, Andreas
AU - Andaleeb, Hina
AU - Bui, Soi
AU - Yefanov, Oleksandr
AU - Catapano, Lucrezia
AU - Henkel, Alessandra
AU - Wiedorn, Max O
AU - Lorbeer, Olga
AU - Crosas, Eva
AU - Meyer, Jan
AU - Mariani, Valerio
AU - Domaracky, Martin
AU - White, Thomas A
AU - Fleckenstein, Holger
AU - Sarrou, Iosifina
AU - Werner, Nadine
AU - Betzel, Christian
AU - Rohde, Holger
AU - Aepfelbacher, Martin
AU - Chapman, Henry N
AU - Perbandt, Markus
AU - Steiner, Roberto A
AU - Oberthuer, Dominik
N1 - © Kara A Zielinski et al. 2022.
PY - 2022/11/1
Y1 - 2022/11/1
N2 - Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance (Klebsiella pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.
AB - Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance (Klebsiella pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.
U2 - 10.1107/S2052252522010193
DO - 10.1107/S2052252522010193
M3 - SCORING: Journal article
C2 - 36381150
VL - 9
SP - 778
EP - 791
JO - IUCRJ
JF - IUCRJ
SN - 2052-2525
IS - Pt 6
ER -