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Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells

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Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells. / Jayson, Christina B K; Arlt, Henning; Fischer, Alexander W; Lai, Zon Weng; Farese, Robert V; Walther, Tobias C.

In: MOL BIOL CELL, Vol. 29, No. 17, 15.08.2018, p. 2045-2054.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Jayson, CBK, Arlt, H, Fischer, AW, Lai, ZW, Farese, RV & Walther, TC 2018, 'Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells', MOL BIOL CELL, vol. 29, no. 17, pp. 2045-2054. https://doi.org/10.1091/mbc.E18-05-0282

APA

Jayson, C. B. K., Arlt, H., Fischer, A. W., Lai, Z. W., Farese, R. V., & Walther, T. C. (2018). Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells. MOL BIOL CELL, 29(17), 2045-2054. https://doi.org/10.1091/mbc.E18-05-0282

Vancouver

Jayson CBK, Arlt H, Fischer AW, Lai ZW, Farese RV, Walther TC. Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells. MOL BIOL CELL. 2018 Aug 15;29(17):2045-2054. https://doi.org/10.1091/mbc.E18-05-0282

Bibtex

@article{7f8a7252164641f883e2241a8cb08152,
title = "Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells",
abstract = "Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the endoplasmic reticulum (ER) but also to lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and function in a human mammary carcinoma cell line. Rab18 localized to the ER and to LD membranes on LD induction, with the latter depending on the Rab18 activation state. In cells lacking Rab18, LDs were modestly reduced in size and numbers, but we found little evidence for Rab18 function in LD formation, LD turnover on cell starvation, or the targeting of several proteins to LDs. We conclude that Rab18 is not a general, necessary component of the protein machinery involved in LD biogenesis or turnover.",
keywords = "Journal Article",
author = "Jayson, {Christina B K} and Henning Arlt and Fischer, {Alexander W} and Lai, {Zon Weng} and Farese, {Robert V} and Walther, {Tobias C}",
year = "2018",
month = aug,
day = "15",
doi = "10.1091/mbc.E18-05-0282",
language = "English",
volume = "29",
pages = "2045--2054",
journal = "MOL BIOL CELL",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "17",

}

RIS

TY - JOUR

T1 - Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells

AU - Jayson, Christina B K

AU - Arlt, Henning

AU - Fischer, Alexander W

AU - Lai, Zon Weng

AU - Farese, Robert V

AU - Walther, Tobias C

PY - 2018/8/15

Y1 - 2018/8/15

N2 - Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the endoplasmic reticulum (ER) but also to lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and function in a human mammary carcinoma cell line. Rab18 localized to the ER and to LD membranes on LD induction, with the latter depending on the Rab18 activation state. In cells lacking Rab18, LDs were modestly reduced in size and numbers, but we found little evidence for Rab18 function in LD formation, LD turnover on cell starvation, or the targeting of several proteins to LDs. We conclude that Rab18 is not a general, necessary component of the protein machinery involved in LD biogenesis or turnover.

AB - Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the endoplasmic reticulum (ER) but also to lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and function in a human mammary carcinoma cell line. Rab18 localized to the ER and to LD membranes on LD induction, with the latter depending on the Rab18 activation state. In cells lacking Rab18, LDs were modestly reduced in size and numbers, but we found little evidence for Rab18 function in LD formation, LD turnover on cell starvation, or the targeting of several proteins to LDs. We conclude that Rab18 is not a general, necessary component of the protein machinery involved in LD biogenesis or turnover.

KW - Journal Article

U2 - 10.1091/mbc.E18-05-0282

DO - 10.1091/mbc.E18-05-0282

M3 - SCORING: Journal article

C2 - 29949452

VL - 29

SP - 2045

EP - 2054

JO - MOL BIOL CELL

JF - MOL BIOL CELL

SN - 1059-1524

IS - 17

ER -