Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells
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Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells. / Jayson, Christina B K; Arlt, Henning; Fischer, Alexander W; Lai, Zon Weng; Farese, Robert V; Walther, Tobias C.
in: MOL BIOL CELL, Jahrgang 29, Nr. 17, 15.08.2018, S. 2045-2054.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells
AU - Jayson, Christina B K
AU - Arlt, Henning
AU - Fischer, Alexander W
AU - Lai, Zon Weng
AU - Farese, Robert V
AU - Walther, Tobias C
PY - 2018/8/15
Y1 - 2018/8/15
N2 - Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the endoplasmic reticulum (ER) but also to lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and function in a human mammary carcinoma cell line. Rab18 localized to the ER and to LD membranes on LD induction, with the latter depending on the Rab18 activation state. In cells lacking Rab18, LDs were modestly reduced in size and numbers, but we found little evidence for Rab18 function in LD formation, LD turnover on cell starvation, or the targeting of several proteins to LDs. We conclude that Rab18 is not a general, necessary component of the protein machinery involved in LD biogenesis or turnover.
AB - Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the endoplasmic reticulum (ER) but also to lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and function in a human mammary carcinoma cell line. Rab18 localized to the ER and to LD membranes on LD induction, with the latter depending on the Rab18 activation state. In cells lacking Rab18, LDs were modestly reduced in size and numbers, but we found little evidence for Rab18 function in LD formation, LD turnover on cell starvation, or the targeting of several proteins to LDs. We conclude that Rab18 is not a general, necessary component of the protein machinery involved in LD biogenesis or turnover.
KW - Journal Article
U2 - 10.1091/mbc.E18-05-0282
DO - 10.1091/mbc.E18-05-0282
M3 - SCORING: Journal article
C2 - 29949452
VL - 29
SP - 2045
EP - 2054
JO - MOL BIOL CELL
JF - MOL BIOL CELL
SN - 1059-1524
IS - 17
ER -