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Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications

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Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications. / Bohnert, Simone; Georgiades, Kosmas; Monoranu, Camelia-Maria; Bohnert, Michael; Büttner, Andreas; Ondruschka, Benjamin.

In: INT J LEGAL MED, Vol. 135, No. 6, 11.2021, p. 2315-2322.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bohnert, S, Georgiades, K, Monoranu, C-M, Bohnert, M, Büttner, A & Ondruschka, B 2021, 'Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications', INT J LEGAL MED, vol. 135, no. 6, pp. 2315-2322. https://doi.org/10.1007/s00414-021-02699-5

APA

Bohnert, S., Georgiades, K., Monoranu, C-M., Bohnert, M., Büttner, A., & Ondruschka, B. (2021). Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications. INT J LEGAL MED, 135(6), 2315-2322. https://doi.org/10.1007/s00414-021-02699-5

Vancouver

Bohnert S, Georgiades K, Monoranu C-M, Bohnert M, Büttner A, Ondruschka B. Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications. INT J LEGAL MED. 2021 Nov;135(6):2315-2322. https://doi.org/10.1007/s00414-021-02699-5

Bibtex

@article{364833b63f6040dabf307f0298a15152,
title = "Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications",
abstract = "The aim of this pilot study was to investigate the diagnostic potential of TMEM119 as a useful microglia-specific marker in combination with immunostainings for phagocytic function and infiltrating capacity of monocytes in cases of lethal monosubstance intoxications by morphine (MOR), methamphetamine (METH), and of ethanol-associated death (ETH) respectively. Human brain tissue samples were obtained from forensic autopsies of cases with single substance abuse (MOR, n = 8; ETH, n = 10; METH, n = 9) and then compared to a cohort of cardiovascular fatalities as controls (n = 9). Brain tissue samples of cortex, white matter, and hippocampus were collected and stained immunohistochemically with antibodies against TMEM119, CD68KiM1P, and CCR2. We could document the lowest density of TMEM119-positive cells in MOR deaths with highly significant differences to the control densities in all three regions investigated. In ETH and METH deaths, the expression of TMEM119 was comparable to cell densities in controls. The results indicate that the immunoreaction in brain tissue is different in these groups depending on the drug type used for abuse.",
author = "Simone Bohnert and Kosmas Georgiades and Camelia-Maria Monoranu and Michael Bohnert and Andreas B{\"u}ttner and Benjamin Ondruschka",
note = "{\textcopyright} 2021. The Author(s).",
year = "2021",
month = nov,
doi = "10.1007/s00414-021-02699-5",
language = "English",
volume = "135",
pages = "2315--2322",
journal = "INT J LEGAL MED",
issn = "0937-9827",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications

AU - Bohnert, Simone

AU - Georgiades, Kosmas

AU - Monoranu, Camelia-Maria

AU - Bohnert, Michael

AU - Büttner, Andreas

AU - Ondruschka, Benjamin

N1 - © 2021. The Author(s).

PY - 2021/11

Y1 - 2021/11

N2 - The aim of this pilot study was to investigate the diagnostic potential of TMEM119 as a useful microglia-specific marker in combination with immunostainings for phagocytic function and infiltrating capacity of monocytes in cases of lethal monosubstance intoxications by morphine (MOR), methamphetamine (METH), and of ethanol-associated death (ETH) respectively. Human brain tissue samples were obtained from forensic autopsies of cases with single substance abuse (MOR, n = 8; ETH, n = 10; METH, n = 9) and then compared to a cohort of cardiovascular fatalities as controls (n = 9). Brain tissue samples of cortex, white matter, and hippocampus were collected and stained immunohistochemically with antibodies against TMEM119, CD68KiM1P, and CCR2. We could document the lowest density of TMEM119-positive cells in MOR deaths with highly significant differences to the control densities in all three regions investigated. In ETH and METH deaths, the expression of TMEM119 was comparable to cell densities in controls. The results indicate that the immunoreaction in brain tissue is different in these groups depending on the drug type used for abuse.

AB - The aim of this pilot study was to investigate the diagnostic potential of TMEM119 as a useful microglia-specific marker in combination with immunostainings for phagocytic function and infiltrating capacity of monocytes in cases of lethal monosubstance intoxications by morphine (MOR), methamphetamine (METH), and of ethanol-associated death (ETH) respectively. Human brain tissue samples were obtained from forensic autopsies of cases with single substance abuse (MOR, n = 8; ETH, n = 10; METH, n = 9) and then compared to a cohort of cardiovascular fatalities as controls (n = 9). Brain tissue samples of cortex, white matter, and hippocampus were collected and stained immunohistochemically with antibodies against TMEM119, CD68KiM1P, and CCR2. We could document the lowest density of TMEM119-positive cells in MOR deaths with highly significant differences to the control densities in all three regions investigated. In ETH and METH deaths, the expression of TMEM119 was comparable to cell densities in controls. The results indicate that the immunoreaction in brain tissue is different in these groups depending on the drug type used for abuse.

U2 - 10.1007/s00414-021-02699-5

DO - 10.1007/s00414-021-02699-5

M3 - SCORING: Journal article

C2 - 34553260

VL - 135

SP - 2315

EP - 2322

JO - INT J LEGAL MED

JF - INT J LEGAL MED

SN - 0937-9827

IS - 6

ER -