Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications
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Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications. / Bohnert, Simone; Georgiades, Kosmas; Monoranu, Camelia-Maria; Bohnert, Michael; Büttner, Andreas; Ondruschka, Benjamin.
in: INT J LEGAL MED, Jahrgang 135, Nr. 6, 11.2021, S. 2315-2322.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications
AU - Bohnert, Simone
AU - Georgiades, Kosmas
AU - Monoranu, Camelia-Maria
AU - Bohnert, Michael
AU - Büttner, Andreas
AU - Ondruschka, Benjamin
N1 - © 2021. The Author(s).
PY - 2021/11
Y1 - 2021/11
N2 - The aim of this pilot study was to investigate the diagnostic potential of TMEM119 as a useful microglia-specific marker in combination with immunostainings for phagocytic function and infiltrating capacity of monocytes in cases of lethal monosubstance intoxications by morphine (MOR), methamphetamine (METH), and of ethanol-associated death (ETH) respectively. Human brain tissue samples were obtained from forensic autopsies of cases with single substance abuse (MOR, n = 8; ETH, n = 10; METH, n = 9) and then compared to a cohort of cardiovascular fatalities as controls (n = 9). Brain tissue samples of cortex, white matter, and hippocampus were collected and stained immunohistochemically with antibodies against TMEM119, CD68KiM1P, and CCR2. We could document the lowest density of TMEM119-positive cells in MOR deaths with highly significant differences to the control densities in all three regions investigated. In ETH and METH deaths, the expression of TMEM119 was comparable to cell densities in controls. The results indicate that the immunoreaction in brain tissue is different in these groups depending on the drug type used for abuse.
AB - The aim of this pilot study was to investigate the diagnostic potential of TMEM119 as a useful microglia-specific marker in combination with immunostainings for phagocytic function and infiltrating capacity of monocytes in cases of lethal monosubstance intoxications by morphine (MOR), methamphetamine (METH), and of ethanol-associated death (ETH) respectively. Human brain tissue samples were obtained from forensic autopsies of cases with single substance abuse (MOR, n = 8; ETH, n = 10; METH, n = 9) and then compared to a cohort of cardiovascular fatalities as controls (n = 9). Brain tissue samples of cortex, white matter, and hippocampus were collected and stained immunohistochemically with antibodies against TMEM119, CD68KiM1P, and CCR2. We could document the lowest density of TMEM119-positive cells in MOR deaths with highly significant differences to the control densities in all three regions investigated. In ETH and METH deaths, the expression of TMEM119 was comparable to cell densities in controls. The results indicate that the immunoreaction in brain tissue is different in these groups depending on the drug type used for abuse.
U2 - 10.1007/s00414-021-02699-5
DO - 10.1007/s00414-021-02699-5
M3 - SCORING: Journal article
C2 - 34553260
VL - 135
SP - 2315
EP - 2322
JO - INT J LEGAL MED
JF - INT J LEGAL MED
SN - 0937-9827
IS - 6
ER -